TLC-densitometric analysis of α-escin in bulk drug substance and in pharmaceutical dosage forms
Journal Title: Current Issues in Pharmacy and Medical Sciences - Year 2015, Vol 28, Issue 3
Abstract
A quite simple and rapid TLC-densitometric method for the identification of α-escin (Aescin) in bulk drug substances was developed. In so doing, different chromatographic conditions, including various mobile and stationary phases, were tested. A TLC-densitometric determination of the examined compound was performed without using visualizing reagent, yet with the use of appropriate dipping reagents, in order to obtain reliable UV-densitometric measurements of α-escin – a substance which has weak chromophore groups. Herein, the application of a mobile phase containing n-butanol-acetic acid-water in volume composition 30:7:13, the use of silica gel 60F254 plates with concentrating zone, and subsequent application of 10% sulphuric acid in ethanol or 5% vanillin in methanol/sulphuric acid, respectively, provided the best results in a TLC-densitometric study of α-escin. The described method was successfully employed to identify α-escin in commercial samples that were in an oral dosage form (tablets) and also in the form of gel containing 20 mg of α-escin.
Authors and Affiliations
Malgorzata Dolowy, Alina Pyka-Pajak, Katarzyna Filip, Joanna Zagrodzka
Keywords
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- EP ID EP112135
- DOI 10.1515/cipms-2015-0069
- Views 95
- Downloads 0
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