TNK1 Deficiency Inhibited Cell Survival, Clonogenic Assay and Effects Expression Levels of EMT Markers
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
PRC is a part of the male reproductive system that helps make and store seminal fluid. In adult men, a typical prostate is about 3 centimetres long and weighs about 20 grams. It is located in the pelvis, below the urinary bladder and in front of the rectum. PRC is considered a malignant tumour because it is a mass of cells that can invade other parts of the body. PRC most commonly metastasizes to the bones, lymph nodes, and may into the rectum, bladder and lower ureters after local progression. The route of metastasis to the bone is thought to be venous as the prostatic venous plexus drairs into the prostate connecting with the vertebral veins. PRC is a zinc-accumulating, citrate-producing organ. The main function of non-receptor protein-tyrosine kinase (nRTKs) is to participate signal transduction in activated T-cells and B-cells in the immune system. Most of the nRTKs are localised in the cytoplasm. TNK1/Kos1 is a 72-KDa NRPTK located on the human chromosome 17p13.1. TNK1/Kos1 is a member of the Ack family, and it is involved in apoptosis and cell growth, but the results are controversial. Nuclear factor-κB and Ras may mediate these effects of TNK1. To investigate the role of TNK1 in PC-3 cells, TNK1 deficient cells were used in the study. The results showed that TNK1 deficiency inhibited cell survival and clonogenicity of PC-3 cells; however, decreased expression EMT markers beta-catenin and vimentin in TNK1 deficient cells. These results suggest that TNK1 might contribute to the maligancy of tumor cells, therefore, TNK1 might be a potential prognosis factor for metastatic prostate cancer.
Authors and Affiliations
Alaulddin Hazim Mohammed
Keywords
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