TRACING OUT OF CHANGES IN THE AMOUNT OF VIABLE MICROORGANISMS GROWING AT 220 C AND 370 C IN BOTTLED NATURAL MINERAL WATERS
Journal Title: Българско списание за обществено здраве (Bulgarian Journal of Public Health) - Year 2013, Vol 0, Issue 3
Abstract
Motivation: In the process off bottling, the conditions, in which mineral water are formed, are change - from open-system it gets into a closed system. Changes were observed in heterotrophic flora, for which, after bottling and during storage of the mineral water, in considerable changes its quantity. It is essential to establish these changes, as it is known that increasing numbers of heterotrophic microflora can be dangerous for seriously ill people or people with compromised immune systems. Objective: Trace amounts of changes in the viable microorganisms growing at 220 C and 370 C in bottled natural mineral water for a period of time / guests. Method: Apply a standard microbiological method - BDS EN ISO 6222. Results: In the present study are traced the changes that occur in abundance of heterotrophic microflora in two brands of Bulgarian bottled mineral water, different in their physical and chemical composition. In one trademark quantity of organisms, growing at 200 ± 20 C and 360 ± 20 C is traced in glass and PET bottles for a period of 97 days, while other mineral water microbial levels were observed up to 63 days in PET bottles. The results show that autochthanous microflora, resulting from the multiplication, reaches levels of 104-105 CFU / cm3 . After a certain period of time the number of the microorganisms stopped to increase and remains more or less constant Conclusion: The reasons that trigger the growth of bacteria in bottled mineral water are the low concentration of dissolved carbon in the mineral water and increased surface of the bottles.
Authors and Affiliations
Vesela Georgieva
Keywords
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