Transcript profiling of vital defense proteins upregulated during 3, 5-Dichloroanthranilic acid (DCA) mediated induced resistance against pearl millet downy mildew disease
Journal Title: International Journal of Life Science - Year 2017, Vol 5, Issue 1
Abstract
Downy mildew disease caused by the biotrophic oomycete Sclerospora graminicola is the main constraint for pearl millet production incurring huge yield and economic losses. The synthetic resistance elicitor 3, 5-Dichloroanthranilic acid (DCA) when applied exogenously as seed treatment to pearl millet at the concentration of 100 μM protected pearl millet plants by inducing systemic and durable resistance against downy mildew. This enhanced resistance correlated with the up regulation of various defense genes and proteins. Transcripts of mRNA of the defense enzymes PAL and POX were significantly enhanced many folds in comparison to the untreated control. The cell wall cross-linking protein HRGPs also showed significant overexpression in DCA treated seedlings compare to the control. Pathogenesis related proteins PR-1 and PR-5 which are regarded as markers of induced resistance were significantly over expressed in DCA treated seedlings, and expression of PR-5 was more than that of the resistant check. Early and increased expression of defense enzymes PAL and POX and defense proteins HRGPs, PR-1 and PR-5 are attributed as causes for enhanced DM protection that is offered by DCA treatment to pearl millet seeds. Changes in transcriptional profiles of defense enzyme sand proteins triggered by DCA clearly resemble typical defense-related responses elicited during elicitor induced resistance suggesting a potential for DCA in management of plant disease in general and pearl millet downy mildew disease in particular.
Authors and Affiliations
Lavanya SN, Niranjan Raj S, Amruthesh KN
Keywords
Related Articles
- EP ID EP541542
- DOI -
- Views 101
- Downloads 0
Seasonal variation in polychaetes of intertidal mangrove area of Shirgaon, Ratnagiri, Maharashtra, India
This study highlights the seasonal variation in polychaetes of intertidal mangrove area of Shirgaon, Ratnagiri, Maharashtra. All polychaetes sample was collected by stratified random sampling method during the lowest low...
Cultivation, Diversity and Bio-Potential of Salix in the Kashmir Himalaya, India
Represented by about 450 species in the world, genus Salix (Salicaceae) is mainly distributed in the temperate regions of the Northern Hemisphere. Salix has been regarded as a platinum crop worldwide and presently its pr...
Modulation of blood profile of juvenile Cyprinus carpio exposed to imidacloprid
Cyprinus carpio is a common fresh water fish abundantly distributed in ponds, beels and canals of India. The fish is regularly consumed because of its high nutritional value. Pesticides are common pollutants of the aquat...
Comparative study to evaluate in vivo and in vitro antioxidant potential of processed and unprocessed Curcuma logna L. using Allxon induced diabetic rats
The present study was aimed to investigate in vivo, in vitro antioxidant activity of the processed curcuma Longa L. turmeric (PT) and unprocessed curcuma Longa L. (UPT). Both the extract are subjected to qualitative phyt...
A simple and reliable method for obtaining monoconidial culture and storage of Magnaporthe oryzae
Monoconidial isolates of Magnaporthe oryzae were successfully cultured and maintained using a simple, reliable and inexpensive isolation procedure developed by us. It can also be performed with minimum equipment. This pr...