Transcriptional activity of genes coding tumour necrosis factor α and its receptors in patients with systemic sclerosis and Raynaud’s phenomenon
Journal Title: Postępy Nauk Medycznych - Year 2012, Vol 25, Issue 10
Abstract
<b>Introduction.</b> The role of TNFα in the pathogenesis of systemic sclerosis (SSc) is still controversial. This factor may play a significant role at the early stage of SSc development, i.e. in vascular changes (Raynaud’s phenomenon – RP) and in fibrosis processes.<br><b>Aim.</b> The aim of this study was to investigate the changes in the number of mRNA copies of genes coding TNFα and its receptors in peripheral blood leukocytes in patients with SSc and RP.<br><b>Material and methods.</b> The research concerned 19 patients with active lSSc, 11 patients with isolated RP and 10 healthy persons. Quantification of TNFα, TNFαRI and TNFαRII genes mRNA was carried out with the use of Quantitative Real-Time Reverse Transcription Polymerase Chain Reaction.<br><b>Results. </b>The number of copies of TNFα mRNA in active lSSc patients was significantly lower than in the control group. No statistically significant difference in transcriptional activity of TNFα gene between active lSSc and isolated RP patients as well as isolated RP and the control group was found. Comparing to controls the expressions of TNFαRI and TNFαRII were significantly lower both in active lSSc patients and in isolated RP patients. The TNFα/TNFαRII ratio both in active lSSc and isolated RP patients was significantly higher opposed to the control group. The TNFαRI/TNFαRII mRNA ratio was significantly higher in patients with active lSSc than in the control group.<br><b>Conclusions.</b> The decrease in transcriptional activity of TNFα, TNFαRI and TNFαRII genes in SSc may be crucial in fibrosis. Receptors proportion may be important in the regulation of TNFα anti-fibrotic activity.
Authors and Affiliations
Anna Lis-Święty, Joanna Gola, Urszula Mazurek, Ligia Brzezińska-Wcisło
Keywords
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