TRANSGENIC (T1) TOBACCO (Nicotiana tabacum) PLANTS OVER EXPRESSING FERRITIN SHOWED TOLERANCE AGAINST OXIDATIVE STRESS
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 1
Abstract
Among different types of Reactive oxygen species, hydroxyl radicle (OH-) is the most toxic and dangerous to the cell and is generated by iron mediated Fenton reaction. Ferritin is a key protein in the iron homeostasis and sequesters iron and makes it non available freely to mediate formation of hydroxyl radicle. With this view, transgenic plants overexpressing ferritin were developed and in the present study T0 seeds obtained from putative transformants were screened on kanamycin for the presence of ferritin gene. Presence of ferritin gene in T1 plants is confirmed by PCR analysis and further leaf discs were exposed to Methyl Viologen induced oxidative stress followed by high light stress to confirm the role of ferritin in oxidative stress. The results indicated that the percent electrolyte leakage and cell damage was higher in wild type plants as compared to transgenic plants. The transgenic plants grew taller and started flowering earlier as compared to wild type plants. Our results indicated that the over expression of ferritin in chloroplast gives tolerance against oxidative stress as compared to wild type plants both at seedling level and at the plant level.
Authors and Affiliations
POORNIMA R, PADMANABHA B V, LOKESHA A N, SHANKAR A G
Keywords
Related Articles
- EP ID EP222815
- DOI -
- Views 103
- Downloads 0
Screening of F1 Hybrids and their Parents for Resistance to Multiple Viruses in Chilli (Capsicum Annuum L.)
Among the viruses infecting chili, potato virus Y (PVY), chili veinal mottle virus (CVMV), tobacco etch virus (TEV), pepper vein banding virus (PVBV), pepper veinal mottle virus (PVMV) belonging to potyvirus group and cu...
TECHNO – ECONOMIC EVALUATION OF TRACTOR OPERATED RAISED BED PLANTERS AND SEED DRILLS FOR CULTIVATION OF WHEAT CROP
A field experiment was conducted to evaluate performance of raised bed planters and seed cum-ferti-drill for cultivation of wheat crop in vertisol at research farm of J.N.K.V.V. Jabalpur (M.P.) during 2013-14. The field...
Design and Development of Low Cost Greenhouse to Raise Different Cultivars”
The cultivar growing technology under the low cost greenhouse may occupy an important role in Indian Agriculture in the future years. The low cost greenhouse ensures the year round growing of different cultivar varieties...
EFFECT OF PLANT GROWTH REGULATORS ON GROWTH AND FLOWERING CHARACTERS OF AFRICAN MARIGOLD (TAGETES ERECTA L.) CV. PUSA NARANGI GAINDA
The Present Investigation Entitled “Effect of Plant Growth Regulators on Vegetative Growth and Flowering Characters of African Marigold (Tagetes Erecta L.) Cv. Pusa Narangi Gainda.” Was Carried out at College of Horticul...
Augmenting the Liquid Waste Management Skills of Rural Shg Women of Madurai District Through Eco-Wash Literacy
Wastewater and solid waste generated due to over population, industrialization and urbanization during these days poses serious environmental pollution problems and improper management of these wastes, in turn threatens...