TRANSGENIC (T1) TOBACCO (Nicotiana tabacum) PLANTS OVER EXPRESSING FERRITIN SHOWED TOLERANCE AGAINST OXIDATIVE STRESS
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 1
Abstract
Among different types of Reactive oxygen species, hydroxyl radicle (OH-) is the most toxic and dangerous to the cell and is generated by iron mediated Fenton reaction. Ferritin is a key protein in the iron homeostasis and sequesters iron and makes it non available freely to mediate formation of hydroxyl radicle. With this view, transgenic plants overexpressing ferritin were developed and in the present study T0 seeds obtained from putative transformants were screened on kanamycin for the presence of ferritin gene. Presence of ferritin gene in T1 plants is confirmed by PCR analysis and further leaf discs were exposed to Methyl Viologen induced oxidative stress followed by high light stress to confirm the role of ferritin in oxidative stress. The results indicated that the percent electrolyte leakage and cell damage was higher in wild type plants as compared to transgenic plants. The transgenic plants grew taller and started flowering earlier as compared to wild type plants. Our results indicated that the over expression of ferritin in chloroplast gives tolerance against oxidative stress as compared to wild type plants both at seedling level and at the plant level.
Authors and Affiliations
POORNIMA R, PADMANABHA B V, LOKESHA A N, SHANKAR A G
Keywords
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