Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration
Journal Title: Revista MVZ Cordoba - Year 2014, Vol 19, Issue 3
Abstract
Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β-glucuronidase (GUS). A. tumefaciens infiltration (agroinfiltration) was made using both leaves from in vitro and 1 month old greenhouse plants. Leaves were incubated in X-GLUC buffer, stained and photographed to detect GUS activity. Results. Agroinfiltration assays showed GUS transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, MCOL2215 (Venezuelan) and CM6438-14 (Vergara), respectively. A. tumefaciens agressive strain AGL1 showed high efficiency inducing GUS expression in cassava leaves. Conclusions. We recommend using A. tumefaciens agressive strain AGL1 for agroinfiltration to assess transient expression in cassava leaves.
Authors and Affiliations
Paula Díaz T, M. Sc| Universidad Nacional de Colombia, Science Faculty, Biology Department, Manihot Biotec Investigation Group, Calle 30 N 45-03, Bogota, Colombia., Adriana Bernal G, Ph. D| Universidad de los Andes, Science Faculty, Biology Department, LAMFU, Cra 1 N 18A- 12, Bogota, Colombia., Camilo López C, Ph. D *| Universidad Nacional de Colombia, Science Faculty, Biology Department, Manihot Biotec Investigation Group, Calle 30 N 45-03, Bogota, Colombia.Correspondence: celopezc@unal.edu.co
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