Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
Journal Title: Journal of Applied Veterinary Sciences - Year 2021, Vol 6, Issue 1
Abstract
A combined vaccine of Equine influenza (EI), equine herpesvirus–1 (EHV-1), rabies virus and tetanus toxoid adjuvanted with saponin and Alhydrogel was prepared. Quality control testing of such vaccine revealed that it was free from foreign contaminants, safe for Guinea-pigs, mice and mares. It was inoculated into Guinea -pigs as a preliminary evaluation for its potency and it was proven safe and potent. Also, it exceeded the permissible protective level allowed to be used for the vaccination of horses. Two groups of mares were used for evaluating the potency of the vaccine. The first one received two doses (5 ml) of the prepared vaccine with 4 weeks interval while the 2nd group was kept without vaccination as control. The mean haemagglutination inhibition (HI) influenza antibody titer reached its maximum at the 2nd month post-vaccination (MPV); 2048 while EHV-1 antibodies reached the peak at the 3rd MPV as recorded by ELISA and neutralizing indices; 1790 and 3.50. Rabies antibodies were detectable in vaccinated mares by the 2nd week post-vaccination showing titers of 16 and 1.0 by serum neutralization test (SNT) and ELISA, respectively recording their peaks (128 by SNT and 2.15 by ELISA) at the 2nd MPV. Tetanus antitoxic titer increased till reaching the peak at the 2nd MPV (40IU/ml) as determined by toxin neutralization test. Depending on these results, it could be concluded that the prepared inactivated EI, EHV-1, rabies and tetanus vaccine is safe and potent for mares providing them with good protective levels of specific antibodies against the 4 used antigens up to 6 months.
Authors and Affiliations
Safaa A. Warda, Eman M. Ebied, Nashwa K. Madkour, Fatma F. Warda, Omaima A. A. Al-Shamandy, Wafaa R. Abdelaziz, Hala El Sawy, and Attyat M. Kotb
Keywords
Related Articles
- EP ID EP692779
- DOI https://dx.doi.org/10.21608/javs.2021.140096
- Views 126
- Downloads 0
Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen
This study aimed to determine the proper sperm dose per laparoscopic intrauterine insemination (LAI) in Zaraiby goats. Thirty-six Zaraiby goats, two Zaraiby and two Boer bucks were used in the study. Does were divided i...
Serological Detection of Newcastle Disease Virus Antibody in Vaccinated and Non-Vaccinated Indigenous Chickens and Guinea Fowls in Atacora and Donga, Northern Benin
Poultry farming is crucial for the livelihoods of small-scale producers in the Northen Benin, yet the industry faces challenges from diseases like ND, which threaten both poultry health and economic stability. This study...
The Immunogenicity And Protective Efficacy Of Dna Vaccine Coding For Na1 Gene Of Highly Pathogenic Avian Influenza H5n1 Subtype
Control of avian influenza infection depends mainly on biosafety measures and vaccination, DNA vaccination is a novel method to generate antigen-specific antibody and cell-mediated immunity. In the current study, a DNA v...
Bovine Fasciolosis in Slaughtered Cattle at Akinyele, Ibadan, Nigeria
Bovine Fasciolosis poses significant public health problems and is of great concern for all livestock farmers. Studies on bovine Fasciolosis have been carried out in different abattoirs in Nigeria. However, there is no s...
Detergent Maceration: A Convenient Skeleton Preparation Technique for Teaching and Demonstration of Veterinary Anatomy
The current study investigates to develop a convenient technique for preparation of a dog skeleton without any unpleasant smell, cracking, crumbling or discoloration of bones by using a combination of commercially availa...