ULTRAFILTRATED FRACTION OF KOREAN RED GINSENG EXTRACT IMPROVES MEMORY IMPAIRMENT OF TG2576 MICE VIA INHIBITION OF SOLUBLE AÎ’ PRODUCTION AND ACETYLCHOLINESTERASE ACTIVITY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 4
Abstract
Objective: The goal of this study was to research for an effective fraction on memory improvement of Korean red ginseng.Methods: In this study, 80 % ethanol red ginseng extract (RE) was divided into inner fluid (REUI) and outer fluid (REUO) by the ultrafiltration and then REUO was further separated into four fractions namely, REUO-00, REUO-30, REUO-50 and REUO-70, respectively, by Diaion HP-20 column chromatography.Results: REUO has protected more significantly the H2O2-induced SHSY-5Y cell death than REUI. Interestingly, the hydrophobic parts of the REUO (REUO-EtOHs) such as REUO-30,-50 and-70 decreased more significantly the H2O2-induced cell death than its hydrophilic part (REUO-00) in a dose-dependent manner. Then, we focused on the activity of a candidate for cholinergic functions, because memory deficits of neurodegenerative diseases are closely associated with cholinergic dysfunctions. The REUO-EtOHs (1.25 mg/ml) inhibited the activity of the acetylcholinesterase and its half maximal inhibitory concentration (IC50) was about 2.358 mg/ml. Additionally, we investigated whether the intake of the REUO (50 mg/kg/d) during 12 w could improve memory impairment of 12-month old Tg2576 mice and decrease total soluble amyloid-β (Aβ) proteins in the mouse brain cortex. The REUO alleviated significantly the memory impairment and successfully reduced the levels of the soluble Aβ proteins in the mouse cortex.Conclusion: We finally suggest that the REUO, including majorly its hydrophobic part that may be considered as more effective for memory improvement, will be highly considered as valuable candidate for the memory-enhancing ingredients against cholinergic dysfunctions and cognitive impairments of neurodegenerative diseases including Alzheimer’s disease.Keywords: Ginseng, Alzheimer’s disease, Acetylcholinesterase, Ultrafiltration, MemoryÂ
Authors and Affiliations
Ki Young Shin, Beom Young Won, Hyun Jee Ha, Yeo Sang Yun, Keun-a Chang, Hyung Gun Lee, Myeong Hwan Oh, Hwan Lee, Young Sik Park, Jong Dae Park, Mi Kyung Pyo
Keywords
Related Articles
- EP ID EP577729
- DOI -
- Views 74
- Downloads 0
CLINICAL OUTCOME OF CALCIUM, VITAMIN D3 & PHYSIOTHERAPY IN OSTEOPOROTIC POPULATION IN THE NILGIRIS DISTRICT
Objective: Osteoporosis and associated fractures are an important cause of mortality and morbidity. The aim of the study is to evaluate the effects of different treatment modalities calcium, calcium+VitD3 and calcium+phy...
DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR METFORMIN HYDROCHLORIDE AND NATEGLINIDE IN BULK AND COMBINED DOSAGE FORM
Objective: To develop an accurate, precise and linear RP-HPLC method for simultaneous quantitative estimation of Metformin hydrochloride and Nateglinide in tablets and validate as per ICH guidelines.Methods: The method u...
NEAR-INFRARED SPECTROSCOPY: A POTENTIAL NEW MEAN OF ASSESSING MULTICOMPONENT POLYHERBAL FORMULATION ON WAY BEFORE AND AFTER EXTRACTION
Objective:Traditional polyherbal medicine and their preparations have been widely used for the thousands of years in developing and developed countries owing to its natural origin and lesser side effects or dissatisfacti...
EYE DROPSWITH NANOPARTICLES AS DRUG DELIVERY SYSTEMS
Objective: The objective of this study was to examine and characterize topical eye drops with indomethacin-loaded poly(vinyl acetate) nanoparticles (IMC-p(VAc)-NPs).Methods: IMC-p(VAc)-NPswere obtained by emulsifier-free...
FORMULATION AND OPTIMIZATION OF ORODISPERSIBLE TABLETS OF IBUPROFEN
Objective: The present study aimed to formulate, develop and optimize orodispersible tablets of ibuprofen.Methods: Orodispersible tablets were prepared by direct compression technique using crospovidone, sodium starch gl...