Ultrasonication Extraction - HPLC-VIS Fluorescence Method Validation for Trace Analysis of Oil and Grease in Water
Journal Title: UNKNOWN - Year 2017, Vol 4, Issue 2
Abstract
A new method was developed to determine the oil and grease in water by HPLC-Vis fluorescence technique. Ultrasonication liquid-liquid extraction method was applied by using 1 ml of toluene to 100 ml of water. The analysis was carried out on a LC-10AT-VP chromatograph with a fluorescence RF. 10 a syknm detector equipped with a varian CN (250 × 4.6 μm) Column. The separation was preformed isocratically using a mixture of 90% solvent (n-Hexane) with 10% solvent (Toluene). Asphaltenic compounds were established to be the main fluorescence chromophores (excitation wave length at 420 nm and emission wave length at 500 nm). The correlation coefficient was (0.992), recovery ranged from 92% to 108% and the limit of detection was found to be 0.06 μg.L-1. The method can be utilized successfully for determination of oil and grease in water samples.
Authors and Affiliations
Mohammed Khaled Elbadawy Babikir
Keywords
Related Articles
- EP ID EP356954
- DOI 10.4172/2380-2391.1000193
- Views 29
- Downloads 0
Phytoremediation: An Environmental Friendly Technique - A Review
Phytoremediation technology is a cost effective and environmentally and ecologically friendly as it utilizes plants natural ability to suck the pollutant present in the soil. There are many plants having this natural abi...
Ultrasonication Extraction - HPLC-VIS Fluorescence Method Validation for Trace Analysis of Oil and Grease in Water
A new method was developed to determine the oil and grease in water by HPLC-Vis fluorescence technique. Ultrasonication liquid-liquid extraction method was applied by using 1 ml of toluene to 100 ml of water. The analysi...
Variations in Nitrogen (NH4+, NO2-, NO3-) and Heavy Metal (Al and Cu)Levels of Water from Swimming Pools in the City Center and Districts of Canakkale, Turkey
In this study, some nitrogen (NO2-, NO3-, NH4+) and metal (Al and Cu) concentrations were analyzed during the summer period by using an ultraviolet-visible (UV-VIS) spectroscopy method in a total of 44 swimming pools loc...
Spatial Distribution of Heavy Metals in Surface and Sub Surface Sediments of the Coastal Area of Kutubdia Island, Cox's Bazar, Bangladesh
Concentrations of selected heavy metals (Zn, Cu, Pb, Cd, Fe, Mn, and Ni) in surface and sub-surface sediments were studied with an attempt to establish a base-line data of their concentration in the sediments of the coas...
Recent Influences of Anthropogenic Activities and Seasons on Heavy Metal Distribution in Shoreline Sediments in Lake Victoria Near Kisumu City, Kenya
The Winam Gulf on Lake Victoria around Kisumu city has seen increase in anthropogenic activities. The activities discharge pollutants, especially heavy metals into the lake. The heavy metals sink into sediments, which sl...