Unraveling the Molecular Diversity of Lipid Degrading Enzymes from Microbial Consortia of a Cold Desert from India
Journal Title: Indian Journal of Cancer Education and Research - Year 2017, Vol 5, Issue 1
Abstract
Metagenomics has emerged as one of the important tools that can endow bottomless insights about the molecular diversity, distribution, ecological roles, microbial grouping and fastidious biological functions of microorganisms in a particular environment. In the present investigation, we explored metagenomics to delineate the molecular diversity of lipid degrading enzymes from one of the cold deserts located at Drass region of Jammu, India. Lipases have been implicated in number of industrial applications that mainly include the food, detergents and bioremediation of the oil contaminated sites. To meet our aim, we used degenerate primers to amplify conserve domain of lipase that encode the catalytic site, from the environmental DNA, followed by pyrosequencing and analysis of the amplified DNA fragments. Interestingly, analysis of the sequencing report revealed diverse classes of lipases from variety of microorganisms, many of which were observed to be unique, having no known representative in the gene database, and henece considered to be novel. The sequenced fragments were aligned and translated into the amino acid sequences, the average length of the translated amino acid sequences were observed to be ~60-100 amino acids that demonstrated~ 40-99% homology with the existing lipases. Phylogeny tree constructed revealed origin and clustering of these lipases. Principle component analysis further confirms the clustering and variations among these sequences, statistically.
Authors and Affiliations
Gupta Puja
Keywords
Related Articles
- EP ID EP462707
- DOI 10.21088/jmrr.2395.6623.3217.12
- Views 83
- Downloads 0
Comparative Study of Folfox 4 Versus Capeox Chemotherapy Regimens in Adjuvant Therapy and Metastatic Colorectal Cancer
Introduction: Adjuvant chemotherapy is considered the standard of care for patients with colorectal cancer after curative resection. Studies on adjuvant and metastatic colorectal cancer showed FOLFOX4 and CAPEOX are equa...
Comparison of Different Phenotypic Tests for Detection of ESBL (Extended Spectrum Beta Lactamases) Producing Pseudomonas Aeruginosa
Background: P. aeruginosa is the most common nosocomial pathogen encountered and a known organism causing high mortality and morbidity. Due to indiscriminate antibiotic use, resistance is very commonly known in them and...
Drug Utilization Review of Anticancer Drugs in Medical Oncology Department of a Tertiary Care Teaching Hospital
The present study aimed to analyze the drug utilization pattern of anticancer drugs in medical oncology department of a tertiary care teaching hospital, Tamilnadu. Each prescription in the department of medical oncology...
Clinical Profile and Outcomes of Acute Myeloid Leukemia: An Institutional Experience from South India
Background: Acute myeloid leukemia is a heterogenous clonal disorder of hematopoietic progenitor cells with different molecular genetic abnormalities, clinical characteristics and variable outcomes with currently availab...
A Multicenterstudyof Solid-Pseudo Papillary Tumor of the Pancreas
We report a retrospective study of Solid Pseudo Papillary Tumor (SPPT) of the pancreas, a rare entity, which is a low grade malignant abdominal tumor at manyinstitute; this is relatively frequent in young women, with a d...