Use of temporary immersion systems for in vitro multiplication of shoots of Anthurium andraeanum Lind. var. Lambada
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 2
Abstract
Two methods to improve the efficiency in the multiplication of shoots of Anthurium andraeanum Lind., they were used to optimize the frequencies of immersion of the explants using a System of Temporary Immersion. The relative efficiency of the method conventional means of culture semisolid for the multiplication of shoots was compared with the method of System of Temporary Immersion. The highest rate of multiplication of shoots was reached using six immersions four times a day during two minutes, controlling the immersion cycles achieves plants better developed and bigger multiplication coefficient. The plantlets multiplied through this method were harvested with 2 to 3 cm of longitude containing from two to five leaves after a period of culture of 50 days. The formation of callus, hiperhidricity and other abnormalities were not observed during the process. The plants obtained by this method were taken to rooting in medium of culture semisólid. This system avoids risks of contamination, it increases yields, it reduces manpower costs and of containers. This technique for its relationship benefit-cost has significant implications for the commercial propagation of the Anthurium.
Authors and Affiliations
Nydia del Rivero Bautista , Elisa Quiala , Daniel Agramante , Raúl Barbón, Wilder Camacho , Lester Morejón, Marta Pérez
Keywords
Related Articles
- EP ID EP684321
- DOI -
- Views 148
- Downloads 0
Detection of endogenous bacterial and fungal contaminants in the establishment of Musa spp.
A method consisted in fixation of microcorm and root of Musa spp. in formaldehyde 10%, inclusion in paraffin and histological cuts, stained differenciality to detect endogenous bacterial and fungal contaminants by inocul...
Effect of filtered bacterial cultures with antifungal activity in vitro in the interaction Musa spp.- Mycosphaerella fijiensis
Although certain bacterial species may be in vitro antagonistic of Mycosphaerella fijiensis, as it was demonstrated, they are discrete results that have their effect on the Musa spp.-M. fijiensis interaction. The resul...
Transgenic plants in the social context
The new biotechnologies take part of the daily life. Within their branches is the vegetal transgenesis which has revolutionized agriculture, considering itself the superior level of the Green Revolution. The transfer of...
Influence of the saline formulation in the conservation of sugarcane plants in vitro at two temperatures
The present work had as aim to determine the saline formulation and its ideal concentration, as well as the culture temperature for the conservation of in vitro plants of sugar cane fran the variaty somaclonal IBP 87-100...
Extraction of total RNA in plants and filamentous fungi
RNA of high quality is a common element required as starting material in the majority of the techniques used in molecular studies either in plants or in phytopathogenic fungi. In this manuscript we emphasized in the ch...