Utility of MPT 64 antigen detection to differentiate between mycobacterium tuberculosis complex and other mycobacteria species
Journal Title: MedPulse -International Medical Journal - Year 2016, Vol 3, Issue 9
Abstract
Background: India has the highest burden of tuberculosis accounting for one fifth of the global incidence. The clinical presentation of pulmonary disease due to nontuberculous mycobacteria (NTM) is similar to that caused by M. tuberculosis complex (MTBC).Accurate diagnosis of tuberculosis is crucial to facilitate early treatment of the patients, and to reduce its spread. Conventional methods are cumbersome and genotypic methods need expertise. A MPT64 TB Ag rapid test has been reported to identify the MTBC from the NTM. The present study was conducted to check the utility of the MPT64 TB Ag test. Material and Methods: A total of 100 mycobacterial isolates recovered from both pulmonary (50 isolates) as well as extra pulmonary specimens (50 isolates) were screened to differentiate the growth of mycobacteria into MTBC and NTM by phenotypic characters and biochemical tests. Further they were tested by MPT64 TB Ag test and results were compared to evaluate the utility of MPT64 TB Ag test. Results: Among 100 mycobacterial isolates, 98 isolates were identified as MTBC and the two isolates from extra pulmonary cases were identified as NTM on the basis of biochemical tests. There was no discrepancy found in differentiation of these isolates between biochemical tests and TB Antigen MPT64 rapid test. Conclusion: The sensitivity and specificity of TB Antigen MPT64 rapid test was 100% as compared to biochemical methods. The MPT64 TB Ag test was found to be a simple, rapid and reliable test to differentiate MTBC from NTM.
Authors and Affiliations
Shripad Taklikar, Prashant Renake, Sujata Baveja, Anjali Swami
Keywords
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