UTILIZATION OF MIXED-SOLVENCY TECHNIQUE IN SPECTROPHOTOMETRIC ANALYSIS OF CEFIXIME TRIHYDRATE TABLETS
Journal Title: Int J of Pharm Res & Analy - Year 2015, Vol 5, Issue 1
Abstract
A novel, economic, eco-friendly, accurate and reproducible method was developed for quantitative analysis of cefixime trihydrate tablets. In present investigation, aqueous blend containing 5% each of sodium citrate, glycerine, PEG300, PEG400, urea and 10% PEG 4000 was used for solubilisation of poorly water soluble drug, cefixime trihydrate, to carry out spectrophotometric analysis. As per statement of Maheshwari (Mixed Solvency concept), each substance (gas, liquid, solid) possesses solubilizing power. The solubility of cefixime trihydrate increased to more than 120 fold as compared to water solubility. Calibration curve of cefixime trihydrate was plotted by noting absorbances of standard solutions (5, 10, 15, 20 and 25 μg/ml) of cefixime trihydrate which were made by diluting stock solution of cefixime trihydrate. The absorbances were noted at 288 nm against respective reagent blanks. No interference of solubilizers was seen above 245 nm. The percentage drug content in two types of marketed tablets was found close to 100 (99.19±1.238 and 100.85±1.821) indicating the accuracy of the proposed method. Recovery studies confirmed accuracy and precision of proposed method. Percentage recoveries estimated by the proposed method ranged from 97.62±1.723 to 99.58±1.073, which are very close to 100. Low values of standard deviation, percentage coefficient of variation and standard error further validated the proposed methods
Authors and Affiliations
Maheshwari RK , Padiyar A, , Putliwala M
Keywords
Related Articles
- EP ID EP127990
- DOI -
- Views 157
- Downloads 0
ISOLATION AND CHARACTERISATION OF MULTI DRUG RESISTANCE STRAINS FROM SKIN OF COWRANCH WORKERS
When drug resistance occurs, the drug or combination of drugs loses its ability to block the germ from reproducing. Over time, the treatment can stop working completely. This means the drugs become less effective....
METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF EZETIMIBE AND GLIMEPIRIDE BY RP-HPLC
ABSTRACT A sensitive, selective and precise high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Ezetimibe and Glimepiride in tablet dosage form. T...
DEVELOPMENT AND VALIDATION OF LORATADINE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV SPECTROSCOPIC METHOD
A simple UV spectroscopic method was developed and validated for the estimation of loratadine in bulk and pharmaceutical dosage form using acetonitrile as solvent. The quantification was achieved at 250nm. Beers la...
DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR DETERMINATION OF ROXITHROMYCIN FROM HUMAN PLASMA
A stable, simple, rapid, precise, accurate HPLC method for analysis of Roxithromycin was developed and validated as per ICH guidelines without need of any internal standard. Separation was carried out using X’terra...
DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR PACLITAXEL IN BULK AND ITS PHARMACEUTICAL DOSAGE FORM
A simple, precise, rapid and accurate reverse phase HPLC method has been developed for the determination of Paclitaxel in bulk and its pharmaceutical dosage form. An enable C18G, 250mm, X4.6mm i.d, 5μm particle siz...