UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To develop a rapid UV spectrophotometric method for the quantitative estimation of Acamprosate calcium (333mg) in tablets and validate as per ICH guidelines.Methods: The optimized method uses a diluent 100% Triethylammonium phosphate buffer (pH 4.0) for the estimation of assay of Acamprosate calcium at a detection wavelength of 208 nm.Results: The developed method resulted in Acamprosate calcium exhibiting linearity in the range 30-90μg/ml. The precision is exemplified by relative standard deviation of 1.5%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) were found to be 99ng/ml, and 300ng/ml respectively. Conclusion: A rapid UV spectrophotometric method was developed and validated for the quantitative estimation of Acamprosate calcium in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
Ashok Kumar Akuthota, A. Sowjanya Kumari, S. Rakesh Reddy, A. Ashok Kumar
Keywords
Related Articles
- EP ID EP579728
- DOI -
- Views 55
- Downloads 0
SYNTHESIS, IN VITRO ANTIMICROBIAL, ANTI-LIVER CANCER EVALUATION OF SOME NOVEL BIS-CYANOACRYLAMIDE AND BIS-AZOLES DERIVATIVES
Objective: Synthesis of some novel bis [3-(aryl)-2-cyanoacrylamide], bis-pyrazole, bis-thiazole and bis-triazole derivatives starting from N,N'-ethane-1,2-diylbis(2-cyanoacetamide) (1) to evaluate for their in-vitro anti...
EVALUATION OF THE GENOTOXICITY OF EURYCOMA LONGIFOLIA AQUEOUS EXTRACT (PHYSTA®) USING IN VITRO AMES TEST AND IN VIVO MAMMALIAN MICRONUCLEUS TEST
Objective: In this study the genotoxic potential of Eurycoma longifolia aqueous extract (PHYSTA®) was investigated by the in vitro Ames test (Salmonella/microsome mutation assay) and the in vivo mouse peripheral blood c...
DESIGN, SYNTHESIS AND MOLECULAR DOCKING STUDY OF HYBRID QUINOLINE-4-YL-OXADIAZOLES/OXATHIADIAZOLES AS POTENT ANTIFUNGAL AGENTS
Objective: The aim of the present work was to design and synthesize hybrid quinoline-4-yl-oxadiazoles/oxathiadiazole derivatives and evaluate them for in vitro antifungal activity against human disease causing pathogens....
ENCAPSULATION OF RED GINGER OLEORESIN (ZINGIBER OFFICINALE VAR RUBRUM) WITH CHITOSAN AS WALL MATERIAL
Objective: This research aims to determine the effect of the spray drying condition against encapsulation efficiency and characterization microcapsules of red ginger oleoresin.Methods: Preparation of encapsulation begun...
COMPARATIVE DOCKING STUDIES ON THE EFFECT OF COMMERCIAL DRUGS ON DIPEPTIDYL PEPTIDASE-4 (DPP-4)
Objective: The aim of our study was to validate the accuracy of computational tools in drug discovery and molecular interaction studies by studying the inhibitory activity of various commercial drugs on DPP-4.Methods: In...