UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS QUANTITATIVE ESTIMATION OF MEBEVERINE HYDROCHLORIDE AND CHLORDIAZEPOXIDE IN CAPSULES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To develop a simple and cheap UV spectrophotometric method for the simultaneous quantitative estimation of Mebeverine hydrochloride (135mg) and Chlordiazepoxide (5mg) in MEVA C Capsules and validate as per ICH guidelines.Methods: The optimized method uses a diluent 100% Triethylammonium phosphate buffer (pH 3.0) for the estimation of assay of Mebeverine hydrochloride and Chlordiazepoxide in Capsules which are analyzed at a detection wavelength of 260nm.Results: The developed method exhibited linearity in the range of 10-40μg/ml for Mebeverine hydrochloride and 2.5-10μg/ml for Chlordiazepoxide. The precision for Mebeverine hydrochloride and Chlordiazepoxide is exemplified by relative standard deviation of 0.499% and 1.75 respectively. Percentage Mean recovery for Mebeverine hydrochloride and Chlordiazepoxide were found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) for Mebeverine hydrochloride and Chlordiazepoxide were found to be 321ng/ml and 3.7ng/ml respectively, while limit of quantitiation (LOQ) for Mebeverine hydrochloride and Chlordiazepoxide were found to be 973ng/ml and 11.2ng/ml respectively. Conclusion: A simple and a cheap UV spectrophotometric method was developed and validated for the simultaneous quantitative estimation of Mebeverine hydrochloride and Chlordiazepoxide in capsules as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
Rajitha Kothapelly, Lakshmi Prasanna Nalluri, Vasundhara Gaddam, Naveen Kumar Rachawar, Ashok Kumar Akuthota
Keywords
Related Articles
- EP ID EP579723
- DOI -
- Views 72
- Downloads 0
KINETIC MODELING OF RASAGILINE MESYLATE FROM NANOSCALE SOLID LIPID PARTICLES
Objective: The present study is aimed to investigate the In-vitro release behavior of the Rasagiline mesylate loaded nanoscale solid lipid particles to provide the quality based controlled release system. Methods: The Ra...
CYTOTOXICITY AND ANTIMICROBIAL PROPERTIES OF NEEM (AZADIRACHTA INDICA) LEAF EXTRACTSCYTOTOXICITY AND ANTIMICROBIAL PROPERTIES OF NEEM (AZADIRACHTA INDICA) LEAF EXTRACTS
Objective: This study was conducted to evaluate the biological properties of Neem (Azadirachtaindica) leaves in terms of its cytotoxic and antimicrobial activities.Methods: The antimicrobial activity of A. indica leaf ex...
FATTY ACID PROFILING AND ANTIOXIDANT POTENTIAL OF TOTAL POLAR LIPID CONTENT OF CYANOBACTERIUM NOSTOC MUSCURUM
Objective: In recent years cyanobacteria has gained much importance due to the presence of secondary metabolites possessing several biological activities like antibacterial, antifungal, anti algal, antiprotozoal, and ant...
DEVELOPMENT OF SUSTAINED RELEASE NANOCAPSULES OF CATECHIN RICH EXTRACT FOR ENHANCED BIOAVAILABILITY
Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its...
APPROACHES TO ACHIEVE AN ORAL CONTROLLED RELEASE DRUG DELIVERY SYSTEM USING POLYMERS: A RECENT REVIEW
The present article contains a brief review of various formulation approaches used in controlled release drug delivery systems, the role of polymers in the controlled delivery of many fast release drugs and the mechanism...