β-Lactam antibiotics promoting aging and clearance of RBCs would deteriorate the DIIHA
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 9
Abstract
Objective To analyze the influence of β-lactam antibiotics on RBC aging and clearance by detecting various indicators of aging and clearance on RBCs, as well as the differences in phagocytosis for erythrocytes before and after drugs treated in vitro. Methods RBCs were treated by β-lactam antibiotics, including Penicillin, Cefepime, Cefoperazone and Ceftazidime, and the changing of phosphatidylserine (PS) and clearance related CD markers, including CD35, CD47, CD55 and CD59 on the surface of the RBCs, were detected by flow cytometry at 0h and 24h after drugs treatment. The proportion of acanthocytes by microscope also at 0h and 24h after drugs treatment was calculated. The phagocytosis of drug-treated RBC was detected by monocyte monolayer assay (MMA). Untreated RBCs were incubated in PBS by the same condition as a negative control.The influence of β-lactam antibiotics on RBC aging and clearance by all the results above was studied. Results Compare to the untreated RBCs, the drug treated RBCs showed a higher PS level on the cell surface. The results showed by percentage as following(0 h vs 24 h): Penicillin 9.42% vs 93.30%, Cefepime 3.88% vs 57.27%, Cefoperazone 4.71% vs 75.75% and Ceftazidime 3.05% vs 43.19%. The acanthocytes ratio was as following(0 h vs 24 h): Penicillin 7.33% vs 86%, Cefepime 2.67% vs 52.67%, Cefoperazone 3.33% vs 67.67% and Ceftazidime 3.33% vs 90.67%. On the opposite, the clearance related CD markers, showed an obviously lower level after drugs treated(0 h vs 24 h): CD35: Penicillin 7.36% vs 11.87%, Cefepime 0.14% vs 28.51%, Cefoperazone 11.85% vs 21.55% and Ceftazidime 7.63% vs 8.73%; CD47: Penicillin 1.22% vs 9.13%, Cefepime 1.80% vs 0.86%, Cefoperazone 0.08% vs 6.85% and Ceftazidime 1.54% vs 5.50%; CD55: Penicillin 14.46% vs 44.31%, Cefepime 17.27% vs 38.41%, Cefoperazone 19.28% vs 33.28% and Ceftazidime 14.62% vs 34.13%; CD59: Penicillin 4.71% vs 20.56%, Cefepime 4.03% vs 7.60%, Cefoperazone 5.91% vs 22.38% and Ceftazidime 5.93% vs 30.89%. Drug-treated RBCs attached more to monocytes than untreated RBCs. Conclusion The β-lactam antibiotics could induce the changing of PS and the clearance of related CD markers on surface of RBCs. They also could lead acanthocytes and make the RBCs more susceptible to phagocytosis by monocytes. The β-lactam antibiotics could promote the RBCs aging and clearance, which might deteriorate the DIIHA.
Authors and Affiliations
Qixiu YANG, Fengyong ZHAO, Qin LI, Jiamin ZHANG, Zhonghui GUO, Ying YANG, Chen WANG, Ziyan ZHU
Keywords
Related Articles
- EP ID EP738339
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.09.005
- Views 42
- Downloads 0
Distribution and molecular basis of A2 subtype in Guangzhou blood donors
Objective To investigate the distribution and molecular basis of A2 subtype among blood donors in Guangzhou. Methods Whole blood samples of 793 group A blood donors in Guangzhou Blood Center were randomly collected. A2 s...
Morphology and proteomic analysis of leukocyte-free apheresis-derived exosome in storage
Objective To investigate the morphological and proteomic differences in exosomes (EXOs) during the storage of leukocyte-free apheresis platelets (LFA-Plt), evaluate the quality of platelets in storage and predict the fun...
Establishment and evaluation of a competitive performance evaluation system for blood centers
Objective To investigate the establishment of a competitive performance evaluation management program for blood centers to ensure the blood supply. Methods A competitive performance evaluation system for Blood Donor Serv...
Clinical observation of auxiliary steel plate combined with autologous platelet rich plasma for the treatment of bone nonunion after intramedullary fixation of long bone fractures
Objective To evaluate the clinical efficacy of utilizing auxiliary steel plates in conjunction with autologous platelet-rich plasma (PRP) therapy for the treatment of bone non-union following intramedullary fixation of l...
Application of traditional teaching combined with PBL in the teaching of transfusion medicine——taking Clinical Blood Transfusion Testing Technique as an example
Objective To explore the application effect of traditional teaching combined with problem based learning (PBL) in the teaching of transfusion medicine taking the chapter of Human Leukocyte Antigen System and Testing in C...