VALIDATED SPECTROPHOTOMETRIC METHOD TO DETERMINE VARDENAFIL AND SILDENAFIL IN PHARMACEUTICAL FORMS USING POTASSIUM IODIDE AND POTASSIUM IODATE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 10
Abstract
Objective: To develop and validate simple, sensitive, precise and free of organic solvents method for the determination of sildenafil (SIL) and vardenafil (VAR) in bulk and pharmaceutical formulation.Methods: The method is based on the reaction of studied drugs with a mixture of potassium iodide and potassium iodate in an aqueous medium at (25±2 °C) to form yellow coloured triiodide ions (I3-) within 45 min. The reaction is followed spectrophotometrically by measuring the absorbance at 288, 351 nm and 285, 351 nm for sildenafil and vardenafil respectively.Results: The effects of analytical parameters on the reported systems were investigated. Beer's law of SIL was obeyed in the range of (0.4-12) μg ml-1and (0.6-16) μg ml-1. Molar absorptivity was found to be (67.659 ×103) lmol/cmand (37.955×103) lmol/cm at 288 nm, 351 nm respectively. Beer's law of VAR was obeyed in the range of (0.2-13) μg/mland (0.5-40) μg/ml. Moreover, molar absorptivity’s were found to be (68.719 ×103) l mol-1 cm-1and (26.691×103) l mol-1 cm-1 at 285 nm, 351 nm respectively.Conclusion: The proposed method has been applied to determine the components in dosage forms with an average recovery of 98.15% to 103.45% and the results have been found in good agreement with those results obtained by the reference methods.
Authors and Affiliations
Amir Alhaj Sakur, Shaza Affas
Keywords
Related Articles
- EP ID EP575449
- DOI 10.22159/ijpps.2017v9i11.20578
- Views 63
- Downloads 0
ANTIDIARRHEAL ACTIVITY OF THYMUS ALGERIENSIS BOISS AND REUT AQUEOUS EXTRACT ON RATS AND MICE
Objective: Thymus algeriensis (Lamiaceae) used in Moroccan popular medicine has been investigated for its antidiarrheal effects.Methods: Antidiarrheal effects of the aerial-part Thymus algeriensis aqueous extract was car...
EVALUATION OF ANTIFUNGAL ACTIVITY OF ORIGANUM VULGARE AND ROSMARINUS OFFICINALIS ESSENTIAL OIL BEFORE AND AFTER INCLUSION IN β-CYCLODEXTRINE
Objective: The aim of this study is to evaluate and compare the antifungal activity of essential oil of Origanum vulgare and Rosmarinus officinalis collected in north region of Albania, and how is it modified by microenc...
THE ESTIMATION OF PHARMACOKINETIC PARAMETERS AND ACCURACY OF THE PREDICTED EQUATION FOR LITHIUM DOSE IN CHILDREN: A PILOT STUDY AT THE YUWAPRASART WAITHAYOPATHUM CHILD PSYCHIATRIC HOSPITAL
Objective: The purpose of the study was to estimate the pharmacokinetic parameters and calculate the precision (%RMSE) of the predicted lithium concentration equation.Methods: This research was studied from blood Lithium...
IN-VITRO COMPARATIVE STUDY OF ANTI-INFLAMMATORY AND ANTI-ARTHRITIC EFFECTS OF FLEMINGIA STRICTA ROXB AND NYMPHAEA NOUCHALI LEAF
Objective: To evaluate the comparative study of anti-inflammatory, anti-arthritic activity of methanol extract of Flemingia stricta and Nymphaea nouchali leaf.Methods: Human Red Blood Cell (HRBC) membrane stabilization m...
DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR ESTIMATION OF CARBAMAZEPINE IN BULK AND TABLET DOSAGE FORM
Objective: To develop and validate simple, accurate, rapid, precise, reproducible and cost effective spectrophotometric method for the quantitative estimation of carbamazepine in a pharmaceutical formulation.Methods: The...