Validation of a Rapid Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection in Dyspeptic Patients-A Study from Central Kerala
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 8
Abstract
ABSTRACT Introduction: Accurate diagnosis of Helicobacter pylori infection in dyspeptic patients is highly essential to institute eradication therapy and to prevent complications. The introduction of ‘test and treat strategy’ using validated rapid stool antigen tests can significantly reduce the burden of H.pylori infection in developing countries. Aim: To validate a rapid monoclonal immunochromatographic stool antigen test, Epituub® fecal H.pylori antigen test kit for the diagnosis of Helicobacter pylori infection among dyspeptic patients. Materials and Methods: Stool samples were collected for Epituub® fecal H.pylori antigen test from randomly selected patients undergoing upper gastrointestinal endoscopy for the evaluation of dyspepsia. Gastric biopsy samples were also collected for urease test and histopathology. The diagnostic criteria for H.pylori infection was defined as a positive test result for both rapid urease test and histopathology examination. All other combination of the results was considered as negative. The test performance was assessed by determining sensitivity, specificity, positive predictive value and negative predictive value with reference to histopathology examination and rapid urease test. Results: Based on the reference criteria, 31% (35/113) patients were diagnosed as H.pylori infected and 41% (46/113) were rapid stool antigen test positive. The sensitivity, specificity, positive predictive value and negative predictive value of the Epituub® fecal H.pylori antigen test kit were 88.5%, 80.76%, 67.3% and 94.02% respectively. Conclusion: Epituub® fecal H.pylori antigen test can be used as a valid alternative to invasive tests for the diagnosis of H.pylori infection. It is also relatively cheap, fast and easy to perform.
Authors and Affiliations
Suryakala Ravi Nair, Prithi Nair Kannambra, Saji K Sebastian
Keywords
Related Articles
- EP ID EP523753
- DOI 10.7860/JCDR/2018/36487.11871
- Views 41
- Downloads 0
Prevalence of Anal Fissure in Patients with Anorectal Disorders: A Single-centre Experience
ABSTRACT Introduction: Anorectal disorders including fissure and hemorrhoids are among the most common digestive complications. To our knowledge there is no literature available on the prevalence of anal fissure from our...
Storage Medium Affects the Surface Porosity of Dental Cements
Introduction: Calcium silicate-based cements physical properties is influenced by environmental changes. Aim: Here, we intended to evaluate the effect of storage medium on surface porosity of root Mineral Trioxide Aggreg...
Biosynthesis, Characterization and Antibacterial Efficacy of Silver Nanoparticles Derived from Endophytic Fungi against P. gingivalis
Introduction: Microbial resistance to existing antimicrobial agents in periodontal therapy is a growing problem. Therefore, there is a need for development of new antimicrobial agents. Aim: To biosynthesize and character...
Hypovitaminosis D is Associated with Gestational Diabetes Mellitus
Introduction: Vitamin D deficiency has been shown to cause expression of insulin gene resulting in high insulin synthesis and secretion. It also decreases insulin resistance, thus affecting abnormal glucose metabolism an...
Students Suicides-Can’t We Stop This?
Letter to Editor