Validation of High Performance Liquid Chromatographic Method for Folic Acid Assay
Journal Title: International Journal of Pharmaceutical Science Invention - Year 2018, Vol 7, Issue 1
Abstract
A high performance liquid chromatography (HPLC) was developed and validated as selective, linear, precise and accurate method for estimation of folic acid (FA). The reversed C18 column (Kromasil 100-5 phenyl® 300 X 4.6 mm, 5µm) was employed at 25°C. The mobile phase was 0.1% v/v trifluoroacetic acid (TFA) and acetonitrile at ratio 80:20 and the flow rate 1.5ml /min. The retention time for folic acid was ± 2.0min. The method showed excellent recovery (99.04%) and precision for determining the folic acid at low concentrations reaching 0.1μg/mL Therefore, the method was accepted as valid stable assay for determination the folic acid in different pharmaceutical dosage forms.
Authors and Affiliations
Eman Sadder El- Leithy, Hend Mohamed Abdel- Bar, Raghda Abd el- Moneum
Keywords
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