VALIDATION OF REVERSED - PHASE HPLC METHOD FOR THE ESTIMATION OF CEFIXIME IN CEFIXIME ORAL SUSPENSION.
Journal Title: International Journal of Pharmacy and Technology - Year 2010, Vol 2, Issue 2
Abstract
A simple, fast, precise and accurate, high performance liquid chromatographic method for estimation of Cefixime in Cefixime Oral Suspension was validated using C18 [Hypersil ODS], 4.6x250mm, 5 microns with mobile phase composition of Tetrabutylammonium hydroxide solution: Acetonitrile (760:240), flow rate of 2.0 ml/min and UV detection at 254 nm. Tetrabutylammonium hydroxide solution was prepared by diluting 25ml of 0.4M Tetrabutylammonium hydroxide solution with water to obtain 1000ml of solution, and pH was adjusted to 6.5 with 1.5M Phosphoric acid. The linearity of the method was observed over concentration range of 140- 260 mcg/ml. The accuracy of the proposed method was determined by recovery studies and found to be 99.17-101.00%. The proposed method is precise, accurate selective, eproducible and rapid for the determination cefixime in cefixime oral suspension.
Authors and Affiliations
Kandikonda. Saikrishna
Keywords
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- EP ID EP107942
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