VALIDATION OF VAGINAL SELF-SAMPLING AS AN ALTERNATIVE OPTION IN PCR BASED DETECTION OF HPV IN CERVICAL CANCER SCREENING IN BOSNIA AND HERZEGOVINA
Journal Title: Genetics & Applications - Year 2017, Vol 1, Issue 1
Abstract
Cervical cancer represents a serious health problem affecting women worldwide especially in developing countries due to low socioeconomic status, inadequate health-care infrastructure, weaknesses in education on this particular issue and lack of effective screening programmes. The primary aim of this study was to assess alternative screening method for the improvement of cervical cancer prevention in conditions of Bosnia and Herzegovina (B&H), which could be applicable in other developing countries as well. The study was conducted on 101 subjects who provided their self-sampled vaginal swabs and/or cervical specimens collected by their gynecologists. Universal Human Papilloma Virus (HPV) primer set optimized to detect a wide range of HPV types was used for HPV genotyping from obtained swab samples in multiplex PCR. Amplicons were analyzed in agarose gel and Agilent 2100 bioanalyzer – a platform based on microfluid technology. Inter-rater agreement kappa (MedCalc2) was used to assess concordance between results of cervical and vaginal sample analysis. Out of 39 subjects who provided their vaginal and cervical samples, results of HPV detection mismatched in 10% of the cases. Inter-rater agreement showed good strength of coincidence between the results of cervical and vaginal sample analysis (kappa=0.748, CI=95 %). We presented an alternative PCR method for the detection of HPV based on vaginal self sampling which is affordable, informative, simple and applicable with high coverage level of defined targeted population and potentially significant in the given cultural and socioeconomic context.
Authors and Affiliations
Ksenija Radić, Lejla Pojskić, Anja Tomić-Čiča, Jasmin Ramić, Daria Ler, Naida Lojo-Kadrić, Naris Pojskić, Kasim Bajrović
Keywords
Related Articles
- EP ID EP349634
- DOI 10.31383/ga.vol1iss1pp23-32
- Views 133
- Downloads 0
Role of host and viral factors and genetic variation of IL28B on therapy outcome in patients with chronic hepatitis C genotype 1b from Serbia
Viral and host factors in hepatitis C virus (HCV) infection can influence on therapy outcome to pegylated interferon/ribavirin (PEG-IFN/RBV) and progression of liver fibrosis. Although novel direct-acting antivirals (DAA...
AN OPTIMIZED DNA ISOLATION PROTOCOL ENABLES AN INSIGHT INTO MOLECULAR GENETIC BACKGROUND OF ENDEMIC Moltkia petraea (Tratt.) Griseb. FROM BOSNIA AND HERZEGOVINA
The Dinaric endemic plant species Moltkia petraea (Tratt.) Griseb. is often called a "living fossil" of ancient Tertiary flora, with great importance for Bosnia and Herzegovina’s biodiversity. Considering its narrow and...
Assessment of Listerine Cool Mint mouthwash influence on possible DNA damage measured by buccal micronucleus cytome assay - preliminary results
Listerine is a brand of mouthwash most used worldwide in oral hygene maintainance. Due to its antimicrobic and antifungal characteristics, it can stop/diminish the development of plaque and gingivitis. Among different ty...
Effects of curcumin and luteolin on viability and cell death induction in NFS-60 cell line
Inducing cell death in tumor cells has been recognized as a promising strategy in curing tumors. Concurrently, interest has been rising for demanding and extensive clinical trials based on the effects of natural products...
MtDNA variation in three main ethnic groups in Tuzla Canton of Bosnia and Herzegovina
This study was based on the analysis of mtDNA polymorphisms in three ethnic groups of Tuzla Canton of Bosnia and Herzegovina (Bosniaks, Croats and Serbs). The main aim of this study was to analyze the influences of the m...