Viability of Clinical Staphylococcus aureus and Staphylococcus aureus ATCC 25923 Under Different Preservation Methods
Journal Title: Journal of Medical Science And clinical Research - Year 2014, Vol 2, Issue 7
Abstract
In this study, four methods of bacterial preservation methods: sterile soil, sterile distilled water, agar overlaid with mineral oil and lyophilisation were employed for maintaining clinical Staphylococcus aureus and Staphylococcus aureus ATCC 25923 for a period of six months (180 days). The morphology, Gram staining properties, biochemical characteristics and viability were monitored at regular intervals of one month (30 days) for six months (180 days).The spread plate colony count method was used to investigate the rate of recovery or viability of all the four organisms from equally inoculated bacterial suspensions (2.0 McFarland equivalent to 8.8 log10 cfu/ml) in differently prepared stock culture of each preservation method. Agar overlaid with mineral oil was found to be the best method for maintenance and preservation of both clinical Staphylococcus aureus and Staphylococcus aureus ATCC 25923 organisms.
Authors and Affiliations
Sulaiman Nna Asma
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