Vitrification affects the expression of matrix metalloproteinases and their tissue inhibitors of mouse ovarian tissue
Journal Title: International Journal of Reproductive BioMedicine - Year 2016, Vol 14, Issue 3
Abstract
Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries. Objective: This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified mouse ovaries. Materials and Methods: In this experimental study, the gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the isolated preantral follicles derived from fresh and vitrified ovaries of 14-16 days old female mice through real time qRT-PCR was evaluated. Developmental parameters, including survival rate, growth, antrum formation and metaphase II oocytes were also analyzed. Results: The developmental parameters of fresh preantral follicles were significantly higher than vitrified preantral follicles. The TIMP-1 and MMP-9 expression levels showed no differences between fresh and vitrified preantral follicles (p=0.22, p=0.11 respectively). By contrast, TIMP-2 expression significantly decreased (p=0.00) and MMP-2 expression increased significantly (p=0.00) in vitrified preantral follicles compared with to fresh ones. Conclusion: Changes in expression of MMP-2 and TIMP-2 after ovarian tissues vitrification is partially correlated with decrease in follicle development.
Authors and Affiliations
Reza Asadzadeh, Shima Khosravi, Saeed Zavareh, Mohammad Taghi Ghorbanian, Seyed Hassan Paylakhi, Seyed Reza Mohebbi
Keywords
Related Articles
- EP ID EP183927
- DOI -
- Views 131
- Downloads 0
Comparison of pregnancy rate between fresh embryo transfers and frozen-thawed embryo transfers following ICSI treatment
Background: The use of assisted reproductive technology (ART) is increasing in the world. The rate, efficacy and safety of ART are very different among countries. There is an increase in the use of intra cytoplasmic sper...
Differential expression of progesterone receptor isoforms related to PGR +331g/a polymorphism in endometriosis: A case-control study
Background: Endometriosis are defined as a progesterone-resistance disease. Two progesterone receptor (PR) isoforms, namely PR-A and PR-B, mediate the special effects of progesterone. One of the most effective polymorphi...
The protective effect of vitamin C on phenylhydrazine-induced hemolytic anemia on sperm quality and in-vitro embryo development in mice
Background: Phenylhydrazine (PHZ) induced anemia and was shown to have harmful effects on the male reproductive system. Objective: To investigate the protective effect of vitamin C (Vit C) on sperm parameters quality, in...
The putative effects of D-Aspartic acid on blood testosterone levels: A systematic review
Background: D-Aspartic acid (D-Asp) is in invertebrate and vertebrate neuroendocrine tissues, where it carries out important physiological functions. Recently, it has been reported that D-Asp is involved in the synthesis...
Aphrodisiac property of aqueous and methanolic extracts of Raphia vinifera (Arecaceae) in sexually experienced male rats
Background: Raphia vinifera (Arecaceae) is a medicinal plant commonly used as a sexual enhancer. Objective: To investigate the aphrodisiac potential of aqueous extract (AE) and methanolic extract (ME) of R. vinifera in s...