Вплив модифікованого живильного середовища на мікроклонування рослин in vitro рижію ярого
Journal Title: Збірник наукових праць Уманського національного університету садівництва - Year 2018, Vol 1, Issue 92
Abstract
Camelina sativa is a promising agricultural crop which has food, technical and energy significance. A short period of vegetation, resistance to diseases and pests and unpretentiousness to the cultivation conditions determine the high economic efficiency of its production. The limiting factor of expanding areas for this crop is lack of high-yielding varieties. Recently, biotechnological methods, in particular microclonal propagation, are used in selection and genetic studies and seed production. The effectiveness depends on a number of factors, the main of which are the composition of the nutrient medium, concentration and ratio of growth regulators in it. This question is poorly known for camelina. In our studies, basic nutrient media were modified by growth regulators of auxin (2.4-dichlorophenoxyacetic acid) and cytokinin (6-benzylaminopurine) nature in various concentrations and ratios. Explants were apical meristems of camelina sprouts of Stepnoi 1 variety. In the course of conducted studies, it is found that a horizontal type of propagation is characteristic for camelina. The biomaterial propagation is due to the development of lateral adventive shoots. For intensive propagation of in vitro plant material of camelina the presence of auxins and cytokinins in the growing medium is a prerequisite. The highest propagation rate (9.3 micro shoots) was observed on the Murasige-Skoog growing medium with the modification of ІАА and 6-BAP at concentrations of 1.0 mg/l. The increase in cytokinin content to 1.5 mg/l slightly changes indicators of the morphogenetic activity. On the Gamborg and Schenk-Hildebrant growing media at the indicated ratio of growth regulators in vitro propagation rate was 26–31% lower. The content and ratio of growth regulators in the growing medium influence mostly the intensity of micro propagation of сamelina sativa in vitro.
Authors and Affiliations
А. І. Любченко, І. О. Любченко, Л. О. Рябовол
Keywords
Related Articles
- EP ID EP523339
- DOI -
- Views 99
- Downloads 0
Визнання та оцінка продукції сільськогосподарського виробництва як особливого об’єкту бухгалтерського обліку, аналізу та планування
Economic aspects of the inclusion of industry products to the index of agricultural production will allow to classify and reflect it in accounting and reporting correctly. The essential aspect in this case is not the tec...
Енергетична ефективність вирощування квасолі звичайної (Phaseolus vulgaris l.) при зрошенні в умовах Південного Степу України
Legumes from the so-called niche cluster are especially popular now, they are chickpeas, lentils, mung beans and haricot beans, which fully satisfy human needs. Haricot beans can not only form high grain yields under con...
Агропродовольча інтеграція у секторі виробництва тваринницької продукції: стан, тенденції, наслідки
The material base of agroindustrial integration is the detailed public division of labor on the fundamentals of specialization and cooperation. It is based on the action of fundamental economic laws - competition, increa...
Оцінка впливу доходів споживачів на обсяги придбання органічних продуктів
For the last two decades, global organic production has been developing dynamically. The largest consumption of organic products per capita in 2015 was recorded in Switzerland (USD 291, EUR 262), Denmark (USD 212, EUR 19...
Вплив гербіциду і біологічних препаратів на динаміку вмісту хлорофілів у листках нуту
The end of the twenty and the beginning of the twenty first centuries is characterized by searching ways to preserve the environment and implement the technologies of biologization of growing an agricultural crop. The de...