Water Extract of Bushen Huoxue Compound Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/b-Catenin Signaling Pathways in Ovariectomized Rats
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2017, Vol 7, Issue 5
Abstract
Bushen Huoxue Compound is a Chinese herbal formula that shows promise in the treatment and prevention of osteoporosis (OP), but the mechanisms underlying its anti-osteoporotic effects are yet to be established. Here, we detect the influence of the extract on several targets of the Notch and Wnt/b-catenin signaling pathways in bone tissue of ovariectomized rats as a model of OP with the aid of real-time PCR, western blot, and immunohistochemical staining. The water extract of BHC exerted a pronounced beneficial effect on OP rats and activated the Wnt/b-catenin pathway through regulating Wnt3a, Wnt10b protein, and glycogen synthesis kinase 3b (GSK-3b) mRNA. Furthermore, the BHC extract inhibited Jagged 1,2 protein and Notch 1,2 mRNA of the Notch signaling pathway. We detected simultaneous changes in the levels of a number of downstream transcripts, including increased runt-related transcription factor 2 (Runx 2) and decreased peroxisome proliferator-activated receptor b (PPARb ), which were closely correlated with the treatment effect. Based on the collective findings, we propose that the water extract of BHC regulates OP-associated factors through modulation of key transcripts and proteins in the Notch and Wnt/b-catenin pathways. Our results provide further mechanistic evidence supporting the clinical efficacy of BHC in the treatment of OP
Authors and Affiliations
Yingquan , Xiong, Hengrui Liu, iefang Wang, Lu Xiang, Zhidi Wu, Haixia Wang, Xiaoyun Li, Xiaoguang Liu, Haibin He, Bojia Peng, Ronghua Zhang
Keywords
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