Water extract of Osmanthus Fragrans attenuates TGF-?1-induced lung cellular fibrosis in human lung fibroblasts cells
Journal Title: International Journal of Pharmacological Research - Year 2015, Vol 5, Issue 8
Abstract
Introduction: Idiopathic interstitial pneumonia, also known as idiopathic pulmonary fibrosis, is a type of diffuse parenchymal lung disease of unknown etiology. Osmanthus Fragrans, referred to as sweet olives, possesses various functions, including neuro-protection, the scavenging of free radicals, and anti-oxidation. Transforming growth factor beta (TGF-?) is a vital growth factor that is essential for regulating cell growth, differentiation, immune-regulation, and extracellular matrices. This study investigates the mechanism underlying water extract of Osmanthus fragrans on the regulation of pulmonary cellular fibrosis. Materials and Methods: The HFL-1 cells, human lung fibroblasts, were cultured with 5 ng/mL of TGF-?1 for 24 h. After the induction of cellular fibrosis, 200 ?g/mL water extract of Osmanthus fragrans was added and cultured for another 24 h. Subsequently, the enzyme-linked immunosorbent assay was performed to quantify the extracellular fibronectin, and Western blot analysis was conducted to examine the expression of TGF-?1 and its downstream SMAD proteins. Results: The findings indicated that 200 ?g/mL of water extract of Osmanthus fragrans significantly attenuates TGF-?1-induced increase in fibronectin. In addition, Osmanthus fragrans dramatically suppresses TGF-?1-induced increases in the expression of pSmad2/3. Conclusion: We propose that water extract of Osmanthus fragrans is a potential fibrosis antagonist for lung fibroblasts. Thus, water extract of Osmanthus fragrans may exert its beneficial effects through suppressing post-receptor signaling of TGF-?1 and restoring lung fibroblasts cells character by blocking the expression of pSmad2/3.
Authors and Affiliations
Yu-Lin Yang, Shu-Jhen Ma, Shu-Fen Liu, Pei-fang Hsieh
Keywords
Related Articles
- EP ID EP434569
- DOI 10.7439/ijpr.v5i8.1951
- Views 82
- Downloads 0
Efficacy and safety of oral vitamin D (cholecalciferol) supplement therapy in Stage I primary hypertension; a prospective, randomized, double-blind, placebo controlled, clinical trial in a tertiary care hospital in central India
Introduction: Hypertension is one of the most common cardiovascular disorders. Almost all targets in its therapy are exploited and there is a growing need to search for newer targets. Vitamin D is gaining increasing atte...
Immunomodulation Potential of Andrographis Paniculata and Tinospora Cordifolia Methanolic Extracts in Combination Forms
Objective: This work mainly focuses on the immunomodulatory effect of different drug combinations (DC-I to DC-V) of methanolic extracts of aerial parts of Andrographis paniculata (AP) and mature stems of Tinospora cordif...
Implementing Standardised Rhodes Index to measure the Efficacy of ginger extract (Zingiber officinale) in pregnancy induced nausea and vomiting
Background: To estimate the efficacy of ginger extract (Zingiber officinale) in pregnancy induced nausea and vomiting. Methods: A total of 30 women with pregnancy of 4-16 weeks, suffering from nausea and vomiting were i...
Factors behind failure of unsupervised self induced medical abortion : A Study from North India
Introduction: Unsafe abortions contribute to 8% of maternal mortality. Easy availability and rampant use of medical methods of abortion lead to upsurge in self induced abortion related complications. Present study was co...
Hepatoprotective Activity of Petroleum ether Extract of Mitragyna inermis
Petroleum ether extract of Mitragyna inermis was studied for hepatoprotective activity against Swiss albino rats with liver damage induced by carbon tetrachloride(CCl4). Results were compared to the standard drug silymar...