Whole blood concentration of thiamine diphosphate requires correction for red blood cell count in the nutritional assessment of thiamine status
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 3
Abstract
This study examined whether the whole blood (WB) concentration of thiamine diphosphate (TDP) needed to be corrected for hemoglobin concentration (Hb) or red blood cell (RBC) count. TDP levels in whole blood (WB TDP) and Hb concentrations and RBC counts were measured in 131 volunteers including subjects with anemia. WB TDP concentrations were more significantly correlated with the RBC count than the Hb concentration. Although the TDP/Hb and TDP/RBC values were significantly correlated with the WB TDP concentrations in the concordance studies, 14 subjects gave a discordant evaluation of thiamine status between assessment by WB TDP concentration and assessments by TDP/Hb value. Six of them revealed a discordant evaluation of the thiamine status between assessment by the WB TDP concentration and by the TDP/RBC value. All subjects with a lower WB TDP concentration presented with lower values of both TDP/Hb and TDP/RBC with or without the presence of anemia. While, the discordant cases were exclusively found in subjects having a normal WB TDP concentration (29-34 ng/mL: reference value, ≧29 ng/mL) but with lower values of TDP/Hb or TDP/RBC. Because these discordant cases were very often observed in correction by Hb concentration, the use of TDP/RBC values was recommended. We may conclude that the correction of the WB TDP concentration by the RBC count is necessary for the nutritional assessment of the thiamine status.
Authors and Affiliations
Hiroshi Ihara, Toshikazu Suzuki, Naotaka Hashizume
Keywords
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