Występowanie i rola aktywnej infekcji wirusem brodawczaka ludzkiego (HPV) w rakach płaskonabłonkowych głowy i szyi
Journal Title: Polski Przegląd Otorynolaryngologiczny - Year 2012, Vol 1, Issue 3
Abstract
HPV, mainly HPV type 16 and to a lesser extent type 18, is a newly identifid causal factor for SCCHN. The association between HPV and SCCHN is strongest for cancers of the tonsil, intermediate for the rest of the oropharynx, and weakest for the oral cavity and larynx. However, The prevalence of HPV in SCCHN in Poland reported in the literature varies from 0–72% One of the reasons of such a different results may be in our opinion method-related false positive results. The aim of the study, was the assessment of HPV prevalence in the head and neck cancer patients in polish population of SCCHN patients. The material comprised of tumor tissue obtained from 50 HNSCC patients with a primary tumor in the oral cavity, oropharynx or larynx, who were scheduled for surgical treatment. For all cases algorithm was performed: First p16 immunostaining was conducted and secondly the HPV DNA has been detected with the use of HPV DNA general primer (GP)5+/6+ PCR, followed by RLB hybridization. In 8% of cases the staining was observed in 50% of cancer cells or more. There was no HPV DNA detected in any case. There was no HPV DNA detected in any case, which may indicate the lower HPV prevalence in polish population than in Western Europe and US and smoking and drinking associated carcinogenesis. The p16 expression has been found in 8% of the patients and supplementary (GP)5+/6+ PCR did not detect the presence of HPV DNA. Thus, the p16 immunostaining is of limited use as a sole method of HPV detection and needs to be complemented by other techniques.
Authors and Affiliations
Paweł Golusiński, Katarzyna Lamperska, Boudewijn Braakhuis, Peter Snijders, Jakub Pazdrowski, Piotr Pieńkowski, Łukasz Łuczewski, Wojciech Golusiński
Keywords
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