XANTHINE OXIDASE INHIBITORY ACTIVITY OF EXTRACTS PREPARED FROM JUNIPERUS PHOENICEA
Journal Title: International Journal of Pharmacognosy - Year 2018, Vol 5, Issue 6
Abstract
The inhibitory effect of Juniperus phoenicea leaves extracts on Xanthine Oxidase activity was evaluated spectro-photometrically at 290 nm. Extracts of Juniperus phoenicea showed a potent XO inhibitory activity. In order to isolate the active compounds, an in vitro bio-guided fractionation was undertaken by preparative chromatographic techniques. Fractions 2, 3 and 6 of ethyl acetate extract demonstrated substancial XO inhibitory activity (>80% inhibition) at 400 μg/mL. The highest inhibitory effect was obtained for the fraction 3 (87.8%). The major constituents amentoflavone (1) and catechin (2) isolated from the fraction 3 were identified by extensive spectroscopic studies including 1D and 2D NMR experiments. These two compounds have no inhibitory effect on xanthine oxidase, it is deduced that the XOI activity of the fraction 3, was due either to a minor bioactive compound or possible synergisms. However this study showed that the tested plant species is potential source of natural XO inhibitors that can be developed, upon further investigation, for treatment of gout and other XO-related disorders.
Authors and Affiliations
Amira Mansour et al.
Keywords
Related Articles
- EP ID EP431994
- DOI -
- Views 82
- Downloads 0
ALTITUDE HEALTH PROBLEMS AND THEIR REMEDIES
People, who have visits to high altitudes, do experience certain type of health problems as they transits to different altitude ranges. With ascend to high altitude, various kinds of acute and chronic physiological chang...
NATURAL REMEDIES TARGET DIFFERENT THERAPEUTIC PATHWAYS IN ORAL MUCOSITIS INDUCED BY CANCER CHEMO OR RADIOTHERAPY
Oral mucositis (OM) is an inflammatory condition affecting oral mucosa. The etiology of this type of inflammation is associated with exposure to radiotherapy or chemotherapeutic drugs. Herbal drugs have been used to indu...
DETERMINATION OF MEXILETINE IN PHARMACEUTICAL PREPARATIONS BY LINEAR SWEEP VOLTAMMETRY
A simple and rapid method for the determination of mexiletine in pharmaceutical preparations was developed and validated using linear sweep voltammetry (LSV). The proposed method was based on electrochemical oxidation of...
HYPOGLYCEMIC AND ANTIHYPERGLYCEMIC EFFECT OF LEAVES EXTRACTS OF PSIDIUM GUAJAVA IN NORMOGLYCEMIC AND STREPTOZOTOCIN-INDUCED DIABETIC MICE
Diabetes mellitus is a metabolic disorder of multiple etiologies characterized by chronic hyperglycemia. As morbidity and mortality rate from diabetes is increasing on one hand and the cost of modern medicine is getting...
GYMNEMA SYLVESTRE R. Br: A COMPREHENSIVE REVIEW ON PHYTOCHEMICAL AND ANALYTICAL STUDY
Gymnema sylvestre R. Br is a valuable herb extensively used in the ayurvedic and indigenous medicinal system, belonging to the family Ascelpiadaceae. It has property of abolishing the taste of sugar and hence given name...