Scientific Journal of Microbiology

Scientific Journal of Microbiology

Basic info

  • Publisher: Sjournals
  • Country of publisher: iran, islamic republic of
  • Platform/Host/Aggregator: Sjournals
  • Date added to EuroPub: 2017/Feb/20

Subject and more

  • LCC Subject Category: Genetics, Immunology, Microbiology, Molecular Biology
  • Publisher's keywords: Microbiology, Molecular, Genetics
  • Language of fulltext: english

Publication charges

  • Article Processing Charges (APCs): Yes 100 EUR
  • Submission charges: No
  • Waiver policy for charges? Yes

Editorial information

Open access & licensing

  • Type of License: Other
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 2012
  • Does the author retain unrestricted copyright? True
  • Does the author retain publishing rights? True

Best practice polices

  • Permanent article identifier: DOI
  • Content digitally archived in: LOCKSS
  • Deposit policy registered in: None

This journal has '82' articles

Bio-efficacy of different antibacterial antibiotic, plant extracts and bioagents against bacterial blight of soybean caused by Pseudomonas syringae pv. glycinea

Bio-efficacy of different antibacterial antibiotic, plant extracts and bioagents against bacterial blight of soybean caused by Pseudomonas syringae pv. glycinea

Authors: G.P. Jagtap| Department of Plant Pathology, Marathwada Krishi Vidyapeeth, Parbhani ,Maharashtra, India, S.B. Dhopte| Department of Plant Pathology, Ma...
( 47 downloads)
Abstract

An experiment was carried out to study efficacy of different antibacterial antibiotic, plant extracts and bioagents against bacterial blight of soybean caused by Pseudomonas syringae pv. glycinea. The results revealed that all the five antibiotics tested in vitro applying poisoned food technique against Pseudomonas syringae significantly inhibited the growth of the test pathogen over untreated control. However, antibiotic, Streptocycline + Copper oxychloride recorded minimum mean colony diameter (10.47mm) and maximum mean inhibition (83.65 mm) of growth of the test pathogen over untreated control (mean col. dia. 90.00 mm and mean inhibition, 0.00) followed by the antibiotic Streptocycline 100 ppm (mean col. dia., 15.64 mm and mean inhibition, 76.69%) and Copper oxycloride (mean col. dia., 21.42 mm and mean inhibition, 62.74%). In field, the highest mean per cent disease incidence 35.51 per cent was observed in poushamycin treatment. The lowest mean per cent disease incidence 12.74 per cent was found in treatment streptocycline 100 ppm + Copper oxychloride (@0.25%) and recorded highest seed yield (2605 kg/ha) and test weight (14.33 g). Plant leaf extract (@ 5 and 10%) of Neem recorded lowest mean colony diameter (34.72 mm) and highest mean mycelial growth inhibition (59.26%) of the test pathogen over untreated control followed by Ginger (mean col. dia, 44.42 mm and mean inhibition, 48.55%).

Keywords: Bio-efficacy, Fungicides, Plant extracts, Bioagents, Mycelial growth inhibition, Glycine max
Management of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum

Management of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum

Authors: G.P. Jagtap| Department of Plant Pathology, Marathwada Krishi Vidyapeeth, Parbhani ,Maharashtra, India, A.M. Jangam| Department of Plant Pathology, Ma...
( 51 downloads)
Abstract

An experiment was carried out to see the efficacy of different chemicals and bioagents against bacterial blight disease severity (PDI) and disease incidence (PI). Significantly low disease severity and low disease incidence were recorded in treatment T4 i.e. copper oxychloride 0.25 % + streptocycline 100 ppm sprays to the tune of 11.83 per cent (PDI) and 19.36 per cent (PI) respectively as against the unsprayed control 27.56 per cent and 45.51 per cent respectively and obtained significantly higher seed cotton yield 2567.33 kg/ha followed by carbendazim 0.1 % + streptocycline 100 ppm. Amongst the antagonist tested against Xanthomonas axonopodis pv. malvacearum, Trichoderma hamatum was significantly superior in per cent reduction of mycelia colony diameter (mm) of pathogen at all the incubation periods tested. The next best antagonist noticed was T. harzianum and P. fluorescens. Per cent reduction in colony diameter (mm) of the pathogen after 3, 6 and 9 days of incubation in T. hamatum was noticed to the tune of 50.94, 48.03 and 44.12 per cent, respectively.

Keywords: Management, Xanthomonas axonopodis pv., Malvacearum, Gossypium spp, Chemicals, Bioagents
Mycological investigation in patients with acute otitis media

Mycological investigation in patients with acute otitis media

Authors: O.J. Akinjogunla*| University of Uyo, Uyo, Faculty of Science, Department of Microbiology, Akwa Ibom State, Nigeria, N.O. Eghafona| Department of Micr...
( 45 downloads)
Abstract

Middle ear swabbed samples from 272 patients with acute otitis media (AOM) attending Ear, Nose and Throat clinics were collected and cultured using standard mycological technique. Deoxyribonuclease and In-vitro susceptibility of the fungal isolates to Flucytosine (Flu), Nystatin (Nys), Voriconazole (Vor) and Ketoconazole (Ket) were evaluated using DNase agar and disc diffusion techniques, respectively. The results showed the highest prevalence of AOM in age group ≤ 10 years with 84 (30.9%) cases and lowest prevalence in age group ≥ 61 having 12 (4.4%) cases. Only 124 (45.6%) samples showed positive growth, while 148 (54.4%) showed no growth. Of the 124 samples with growth, 9(36.4%) samples showed growth of single fungal isolate, while 18 (6.6%) and 7 (2.6%) showed growth of two and three fungal isolates, respectively. Aspergillus niger was the predominant fungal isolate, followed by Candida albicans with 38 (24.4%), Cryptococcus neoformans 32 (20.5%), Candida spp 21 (13.5%) and Aspergillus flavus 14 (9.0%). Only 46 (29.5%) of fungal isolates were deoxyribonuclease producers, with 30/38 (78.9%) being C. albicans and 16/21 (76.2%) being Candida spp. The results of antifungal susceptibility showed that between 65.8 to 71.1% C. albicans, 64.3 to 85.7% A. flavus, 64.7 to 78.4% A. niger, 53.1 to 75.0% C. neoformans, and 52.4 to 57.1% Candida spp. were sensitive to Ket, Vor and Flu, while between 81.6-84.4% of C. albicans and C. neoformans were sensitive to Nys. In overall, 72.4%, 76.3%, 67.9% and 62.2% of the fungal isolates were sensitive to Flu, Nys, Vor and Ket, respectively. Consequently, Nystatin will be highly effective in treating AOM caused by fungi.

Keywords: Acute otitis media, Deoxyribonuclease, Antifungal, Prevalence, Fungi
Evaluation of natural plant extracts, antagonists and fungicides in controlling root rot, collar rot, fruit (brown) rot and gummosis of citrus caused by Phytophthora spp. in vitro

Evaluation of natural plant extracts, antagonists and fungicides in controlling root rot, collar rot, fruit (brown) rot and gummosis of citrus caused by Phytophthora spp. in vitro

Authors: G.P. Jagtap| Department of Plant Pathology, Marathwada Krishi Vidyapeeth, Parbhani ,Maharashtra, India, M.C. Dhavale| Department of Plant Pathology, M...
( 59 downloads)
Abstract

The results revealed that all the six bioagents tested in vitro applying dual culture technique against Phytophthora nicotianae, Phytophthora citrophthora and Phytophthora palmivora significantly inhibited the mycelial growth of the test pathogen over untreated control. However, bioagent, Trichoderma harzianum recorded minimum mean colony diameter (7.73 cm2) and highest inhibition (87.85%) of mycelial growth of P. nicotianae over untreated control followed by the bioagent T. viride, T. koningii which recorded mean colony diameter of 9.95 cm2, 14.15 cm2 and mean mycelial inhibition of 84.36 %, 77.76%, respectively. Evaluation of different botanicals by Poisoned Food Technique showed that all plant extracts tested in vitro were found significantly effective in reducing the percentage mycelial growth of P. nicotianae, P. citrophthora and P. palmivora over untreated control. However, plant extract (@ 5, 10 and 15%) of Garlic, recorded lowest mean colony diameter (47.45 mm) and highest mean mycelial growth inhibition (47.26%) followed by Neem, Onion which recorded the mycelial growth of 55.20 mm, 60.85 mm, and the mean mycelial growth inhibition of 38.65%, 32.38%, respectively. Results revealed that all the nine fungicides tested in vitro applying Poisoned Food Technique against P. nicotianae, P. citrophthora and P. palmivora significantly inhibited the mycelial growth of the test pathogen over untreated control. However, fungicide, Cymoxynil 8% + Mancozeb 64% (Curzate M-8) recorded minimum mean colony diameter (16.12 mm) and maximum mean inhibition (82.09%) of mycelial growth of the test pathogen over untreated control (mean colony diameter 90.00 mm and mean inhibition 0.00) followed by the fungicide Metalaxyl-M 4% + Mancozeb 64% (Ridomil Gold), Metyram (Polyram), which recorded mean colony diameter of 20.16 mm, 24.16 mm and mean mycelial inhibition of 77.59%, 73.14%, respectively.

Keywords: Citrus, Phytophthora spp., Plant extracts, Bioagents, Fungicides
Bacteriological and metal analyses of water samples from Awotunde fish pond and river

Bacteriological and metal analyses of water samples from Awotunde fish pond and river

Authors: A.T. Odeyemi*| Department of Microbiology, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria, R.O. Agunbiade| Department of Zoology, Ekiti State...
( 50 downloads)
Abstract

The microbiological and metal analyses of water samples obtained from Awotunde fish pond and river in Ila-Orangun, Osun State, Nigeria were determined. The total bacterial and coliform counts were done using pour plating technique. Mineral was analyzed using Atomic Absorption Spectrophotometry techniques. The physicochemical parameters were done using standard methods. The total bacterial and coliform count of the water samples ranged 6.4 x 104 CFU/ml - 7.4 x 104 CFU/ml and 1.1 x 103 CFU/ml - 4.0 x 103 CFU/ml respectively. The values obtained for the physicochemical parameters ranged as follows: temperature (24 - 26) oC, HCO3 (2.0 - 4.6), pH (6.0 - 7.2), alkalinity (40 - 100) mg/l, hardness (60 - 120) mg/l, conductivity (200 - 400)μS/cm, dissolved oxygen (1.1 - 2.0) mg/l, BOD (0.06 - 0.22) mg/l, chloride (28.4 - 106.4) mg/l, nitrite (4.0 - 188.0) mg/l, sulphate (14.0 - 640.0) mg/l, nitrate (0.0 - 90.0) mg/l and phosphate (1.6 - 83.2) mg/l. The mean values of the mineral contents of the water samples are as follows; potassium (94.4 mg/l), sodium (94.7 mg/l), magnesium (89.3 mg/l) and calcium (94.9 mg/l). The mean value obtained for the heavy metal concentrations are as follow; copper (0.1 mg/l), manganese (0.13 mg/l), nickel (2.09 mg/l), lead (0.03 mg/l) and iron (0.26 mg/l). This shows that heavy metal concentrations were below critical limit according to the WHO standard. However, the microbial counts obtained portend the fact that the fish harvested from the pond might be a source of food poisoning if such fishes are not adequately cooked. Since the river serve as a source of water for domestic usage, an outbreak of water borne diseases could be imminent. Hence there must be a need for the Nigeria fish farmers to improve on their fish farming management which includes fish food preparation, environmental sanitation and monitoring the quality fish yield.

Keywords: Microbiological, Awotunde fish pond, Physicochemical, Coliform, Heavy metal
Effect of agrochemicals on microflora in soybean rhizospheric soil

Effect of agrochemicals on microflora in soybean rhizospheric soil

Authors: G.P. Jagtap| Department of Plant Pathology, Marathwada Agricultual University, Parbhani. Maharashtra, India
( 47 downloads)
Abstract

After application of agrochemicals at 1, 30 DAS and at harvest the total number of fungi, bacteria, actynomycetes, pseudomonas, azatobactor, rhizobium were counted.The result with regard to bacterial population in soybean field were significantly influenced by bioinoculant viz., rhizobium. The bacterial populations were inhibited by herbicides Alachlor in soybean and fungicides i.e. thiram and mancozeb in soybean. After 30 days of spraying of chemicals the bacterial populations were restored. With regard to rhizobium population, the bioinoculant were significantly influenced the population soybean field. The herbicides and fungicides were significantly decreased bioinoculant population, maximum inhibition was observed in mancozeb treated plot at 30 DAS. The results with regards to pseudomonas, actinomycetes and fungi population were influenced by bioinoculant viz., rhizobium. While population were inhibited by alachlor, thiram and mancozeb in soybean field. The Pseudomonas, actinomycetes and fungi population were restored after 30 days of spraying. With regard to yield of soybean were significantly influenced by bioinoculant in combination with herbicide and fungicide.

Keywords: Agrochemicals, Bioinoculants, Soybean
Recurrent appearance of foot and mouth disease virus (FMDV) in Saudi Arabia

Recurrent appearance of foot and mouth disease virus (FMDV) in Saudi Arabia

Authors: A.F. Alsayeqh| Departement. of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, 51354 Buraydah, KSA., S.M. Fat'...
( 67 downloads)
Abstract

There are seven immunologically distinct serotypes of FMDV (O, A, C, Asia 1, SAT 1, SAT 2 and SAT 3), most of them were detected and identified in different regions of Saudi Arabia. FMDV serotype O was the most frequent strain in last decade. Outbreaks of FMDV repeatedly occur among cattle, sheep and goats in various regions of Saudi Arabia during years 1994, 1995, 2000, 2004, 2005, 2006, 2008 and 2010. Recently, 14 (0.78 %) suspected cases of FMDV out of inspected 1800 cows were observed and recorded during Hajj season 1432 H (2011) in Makkah. Prevention and control strategies of FMD in Saudi Arabia particularly during Hajj seasons were discussed.

Keywords: FMD, Recurrent, Appearance, Prevention, Strategy, Outbreaks
In-vitro antibacterial evaluation of ethanolic stem crude extracts of anacardium occidentale linn. (anacardiaceae) on streptococcus mutans associated with dental caries

In-vitro antibacterial evaluation of ethanolic stem crude extracts of anacardium occidentale linn. (anacardiaceae) on streptococcus mutans associated with dental caries

Authors: O.J. Akinjogunla*| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B.1017, Uyo, Akwa Ibom State., I.T. Adenugba| Department of...
( 58 downloads)
Abstract

The preliminary phytochemistry and antibacterial activity of ethanolic stem crude extracts of Anacardium occidentale on Streptococcus mutans isolated from dental caries were determined using chemical, standard microbiological and agar disc diffusion techniques. The phytochemical analysis of the ethanolic stem crude extracts of Anacardium occidentale revealed the presence of alkaloids (+++), phenolics (+++), saponins (+), tannins (++), flavonoids (++), phlobatanins (+), anthraquinones (+), terpenes (++), deoxy-sugar (++) and cardiac glycosides (+). The results showed that between 65.2% to 95.6% of S. mutans isolated were sensitive to different concentrations of ethanolic stem crude extracts of A. occidentale with Activity Index (A.I) ranging from 0.38 to 1.22. Ethanolic stem crude extracts of A. occidentale showed the highest mean zones of Inhibition (17.9 ± 1.3mm) at 50.0 mgml-1 and lowest inhibition diameter (6.5±1.5mm) at 12.5 mgml-1 on S. mutans, thus, exhibiting concentration dependent activity. The results show that S. mutans with code WD09 was resistant to Streptomycin, Amoxycillin, di-methyl sulphoxide and A. occidentale ethanolic stem crude extracts. Therefore, there is a need to consider the use of this potent ethanolic stem crude extracts of Anacardium occidentale that have shown some measures of antimicrobial potency, judging by the antibacterial activity and activity index for developing synthetic drugs against dental caries caused by Streptococcus mutans.

Keywords: Streptococcus mutan, Cashew, Caries, Phytochemical, Antibacterial
Antibiogram of isolated bacteria from Omisanjana hand-dug well water and flowing stream

Antibiogram of isolated bacteria from Omisanjana hand-dug well water and flowing stream

Authors: A.T. Odeyemi*| Microbiology Department, Ekiti State University, Ado-Ekiti. Nigeria., J.O. Oluyege| Microbiology Department, Ekiti State University, Ad...
( 47 downloads)
Abstract

Water samples were obtained from ten (10) wells around Omisanjana stream, Ado-Ekiti to determine the microbiological and physicochemical quality. The samples were analyzed for the total bacterial and coliform count and the isolated organisms were identified using standard techniques which were further screened for susceptibility to various antibiotics commonly used in the community. The total bacterial count ranged from 2.6 x 103 to 10.9 x 104 CFU/ml and the total coliform counts ranging from 3.0 x 102 to 9.3 x104 CFU/ml; which exceeded the WHO standard for drinking water. The organisms isolated belonged to eleven genera, among which Staphylococcus aureus showed highest frequency (24.6%) while Acinetobacter spp. with least frequency (4.3%). The physicochemical properties of the water accord with the WHO standard with pH range of (6.2-71), temperature (26.9-29.2), turbidity (-0.8 - 6.5) NTU, conductivity (0.04-0.23)µS/cm, total hardness (14-80) mgL-1, TDS (34 to166) mgL-1 and TSS of (29 to 122) mgL-1. Higher level of resistance to the antibiotics tested was more prominent in the E.coli than in Staphylococcus aureus. Although some strains were susceptible to some commonly used antibiotics, but the resistant bacteria encountered pose a serious public health risk especially with the increasing rate of transfer of resistant genes from one bacterium to another. There is therefore need to treat water obtained from wells in the community to make it safe for domestic use.

Keywords: Omisanjana stream, Hand dug well, Antibiotic susceptibility, Coliform
Prevalence of brucella abortus antibodies in bovine serum from gusau modern abattoir, Zamfara state, Nigeria

Prevalence of brucella abortus antibodies in bovine serum from gusau modern abattoir, Zamfara state, Nigeria

Authors: N. Lawal*| Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., G.O. Egwu| Faculty of Veterinary Medicine, University of Mai...
( 60 downloads)
Abstract

A study for bovine brucellosis was conducted using serology to determine the status of the disease in slaughtered cattle. Three hundred and twenty (320) sera were collected from Gusau Modern Abattoir, Zamfara State. The sera were tested using Enzyme Linked Immunosorbent Assay obtained from Veterinary Laboratory Agencies, Weybridge, UK. An overall prevalence of 20% (64 positive) was obtained with sex prevalence for males and females being 10.62% (34 positive) and 9.37% (30 positive) respectively out of 180 males and 140 females tested without significant association (P < 0.05). On age distribution, higher prevalence of 11.87% was recorded in age group > 24 months while; lower prevalence of 3.13% was recorded in age group < 12 months. There was no significant association statistically between age and occurrence of antibodies. White Fulani breed had the highest prevalence of 8.75%. There was significant association statistically (p < 0.05) between breed and infection.

Keywords: Abattoir, Brucella abortus, Antibodies, Serum, Elisa, White Fulani
Antibiotic susceptibility profile for salmonella in the Buea health district

Antibiotic susceptibility profile for salmonella in the Buea health district

Authors: A.L. Njunda| Department of Medical Laboratory Sciences, University of Buea, P.O.Box 63, Buea, Cameroon., N.J.C. Assob| Department of Public Health and...
( 44 downloads)
Abstract

Resistance of Salmonella to antibiotics is a serious health problem in developing countries. The aim of this study was to determine the antibiotic susceptibility profile of Salmonella in Buea. Stool samples were collected from 100 patients presenting with symptoms of typhoid and cultured on different media. 21 samples were positive for Salmonella and antibiotic susceptibility testing was done on these samples by the disc diffusion method, using 9 different antibiotics. Out of the 21 samples, 18(85.7%) were sensitive to Ciprofloxacin while Nalidixic acid recorded a high rate of resistance with only 2(9.5%) being sensitive to the drug.

Keywords: Salmonella, Antibiotic, Sensitivity, Resistant, Culture, Agar
Microbial quality of selected sandwiches sold at retail outlets of fast food shops in Dhaka city and Mymensingh town of Bangladesh

Microbial quality of selected sandwiches sold at retail outlets of fast food shops in Dhaka city and Mymensingh town of Bangladesh

Authors: N. Hoque| Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., S.M...
( 43 downloads)
Abstract

the experiment was designed to undertake a study on the sanitary quality of commercial fast foods sold at retail outlets of fast food shops in Dhaka city and Mymensingh town of Bangladesh. One hundred sandwiches were subjected to bacteriological examinations. The mean values of total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) in wrapped and unwrapped samples of pre-microwave oven and post-microwave oven fast foods were determined. In pre-microwave oven the TVC, TCC and TSC were log 6.36, log 3.42 and log 4.21 respectively. similarly in post-microwave oven the TVC, TCC and TSC were log 4.89, log 2.60 and log 3.03 respectively. The role of packaging and heat treatment prior to sale on the bacterial changes of the fast foods were demonstrated. It was observed that the values of TVC of all fast food samples except post-microwave oven treated McDonald’s; Pizza and Ruma were higher than the maximum microbial limit. In TCC and TSC all values were higher than the maximum limit. The degree of initial contamination in fast food samples which may pose health hazard to public health has been discussed. It was concluded that the hygienically packaged fast foods and microoven treatment prior to sale would retain the best quality attributes required for consumer’s acceptability and safety.

Keywords: Sandwiches, Total viable count, Total coliform count, Total staphylococcal count
The antimicrobial activities of Ethanolic extracts of Basella alba on selected microorganisms

The antimicrobial activities of Ethanolic extracts of Basella alba on selected microorganisms

Authors: O.A. Oyewole*| Department of Microbiology, Federal University of Technology. PMB 65, Minna, Niger State, Nigeria., O.A. Kalejaiye| Department of Micro...
( 51 downloads)
Abstract

The antimicrobial effects of ethanolic extract of Basella alba against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albican was determined using the agar cup plate method. The phytochemical components of the ethanolic extracts of the leaf and stem of B. alba showed the presence of tannin, terpene, steroid, saponin, anthraquinone, and with carbohydrate only in the stem extracts. The result of this study showed that all the organisms except Candida albican. were susceptible to 60mg/ml and 100mg/ml. of extract. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. The result obtained showed that the MIC and MBC for the ethanolic extract of the leaf and stem of P. aeruginosa, E. coli was 50mg/ml, while the MIC and MBC of S. aureus was 100mg/ml for the ethanolic extract of the leaf and stem of B. alba. The result of this study suggests that the ethanolic extracts of B. alba could be suitable for the treatment of diseases caused by S. aureus, P. aeruginosa and E. coli.

Keywords: Antimicrobial, Phytochemical, Ethanolic, Susceptible, Minimum inhibitory, Concentration
Gamma irradiation effects on microbial decontamination of ostrich meat

Gamma irradiation effects on microbial decontamination of ostrich meat

Authors: F. Tabatabaei Yazdi*| Department of Food Science and Technology, Ferdowsi University of Mashhad, Iran., M. Jouki| Young Researchers Club - Islamic Aza...
( 91 downloads)
Abstract

In this work effects of gamma irradiation and storage time on microbial decontamination of fresh camel meat stored at 4˚C were evaluated. Microbial analysis indicated that irradiation had a significant effect on the reduction of microbial loads. Among the analyzed bacteria, coliforms were most sensitive to gamma radiation. Considering the sensory analyses and Total bacterial counts analyses as a whole, air-packed samples irradiated at 1.0 kGy were acceptable under refrigerated storage for 9 days, compared to 5 and 7 days for irradiated at 3.0 kGy and non irradiated air-packaged samples, respectively.

Keywords: Gamma irradiation, Shelf life extension, Ostrich meat, Refrigeration
Characterization of Escherichia coli isolates obtained from washing and rinsed water of broilers in pluck shops at Sreepur of Gazipur district in Bangladesh

Characterization of Escherichia coli isolates obtained from washing and rinsed water of broilers in pluck shops at Sreepur of Gazipur district in Bangladesh

Authors: Tuhin-Al-Ferdous| Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Banglade...
( 44 downloads)
Abstract

The study was aimed at characterization of Escherichia coli isolates obtained from washing and rinsed water of broilers in pluck shops at Sreepur of Gazipur district in Bangladesh. A total of 30 samples collected from the different layers of drums of pluck shops’ were subjected to bacterial isolation and identification by using cultural and biochemical techniques. Furthermore, the isolated E. coli strains were characterized by antimicrobial susceptibility testing. The fermentation reaction by the isolates of E. coli in five basic sugars (dextrose, sucrose, fructose, maltose, mannitol) and in dulcitol were positive. Moreover, methyl red reaction and catalase tests were also positive for E. coli. On the other hand, E. coli was prevailed in upper layer of drums (31.82%), in middle layer of drums (36.36%) and in lower layer of drums (31.82%). E. coli isolates were resistant to erythromycin and enrofloxacin. However, most of the E. coli isolates were susceptible to sulfamexazole-trimethoprim and gentamycin. Out of 22 E. coli isolates, 16 (72.73%) were multidrug resistant. The findings of the study revealed the presence of multidrug resistant E. coli isolates in washing and rinsed water of broilers in Pluck shops at Sreepur of Gazipur district in Bangladesh.

Keywords: Characterization, Escherichia coli, Washing and rinsed water of broilers, Pluck shops

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