Journal of Advanced Biotechnology and Experimental Therapeutics

Journal of Advanced Biotechnology and Experimental Therapeutics

Basic info

  • Publisher: Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
  • Country of publisher: bangladesh
  • Date added to EuroPub: 2019/Feb/20

Subject and more

  • LCC Subject Category: Biological Sciences, Genetics, Microbiology, Pharmacology, Toxicology
  • Publisher's keywords: Cellular and Molecular Biology, Genetics and Genomics, Immunology and Physiology, Metabolism and Diseases, Microbiology and Infection, Pharmacology and Toxicology, Plant Biology and Herbal Medicine, Stem Cells and Cancer Biology, Reproductive and Aquatic Biology
  • Language of fulltext: english
  • Full-text formats available: PDF, HTML

Publication charges

  • Article Processing Charges (APCs): Yes 30 USD
  • Submission charges: No
  • Waiver policy for charges? No

Editorial information

Open access & licensing

  • Type of License: CC BY-NC
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 2018
  • Does the author retain unrestricted copyright? True
  • Does the author retain publishing rights? True

Best practice polices

  • Permanent article identifier: DOI
  • Content digitally archived in: Nopolicy
  • Deposit policy registered in: None

This journal has '24' articles

Choice of samples in extracellular microRNA research: Which fraction is better- exosomal or nonexosomal?

Choice of samples in extracellular microRNA research: Which fraction is better- exosomal or nonexosomal?

Authors: Md Mahmodul Hasan Sohel
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Abstract

Abstract: MicroRNAs (miRNAs) are a species of noncoding RNA that post-transcriptionally regulates the expression of target mRNAs via degradation or translational repression. Recently, miRNAs in biofluids got extraordinary research priority in the field of noninvasive biomarker development because of their association with pathophysiological events, noninvasive way to obtain, and are markable stability in the extracellular harsh environment. Although several hypotheses have been proposed, the precise mechanisms of cellular release of extracellular miRNAs are not properly understood. In addition, it is often confusing to the researchers, which fraction of biofluid (based on release mechanisms) is suitable for biomarker development research. This review will briefly introduce extracellular miRNAs (EC miRNAs) and their release mechanisms. Furthermore, based on recent evidence, it will also summarize and suggest the appropriate fraction of biofluid to work with for biomarker development.

Keywords: Circulating microRNA; extracellular microRNA; exosomal; nonexosomal; serum/plasma
DNA fingerprinting and molecular diversity analysis for the improvement of brinjal (Solanum melongena L.) cultivars

DNA fingerprinting and molecular diversity analysis for the improvement of brinjal (Solanum melongena L.) cultivars

Authors: Sharmin Sultana, Mohammad Nazrul Islam, Md Ekramul Hoque
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Abstract

An investigation was conducted to assess genetic diversity of Bangladesh Agricultural Research Institute (BARI) released 11 varieties of brinjal (BARI begun 1, BARI begun 4-10, BARI hybrid begun 1, 3 and 4) using PCR-based randomly amplified polymorphic DNA (RAPD) markers. A total of 44 distinct DNA amplified bands were observed for five primers (OPB-04, OPB-08, OPD-02, OPP-13 and OPW-08) with an average of 5.6 polymorphic bands per primer. The overall gene diversity was detected 0.216 and level of polymorphism was found 63.64%. The pair wise inter-variety similarity indices ranged from 67.03% to 97.61%. The UPGMA dendrogram segregated 11 genotypes of brinjal into two main clusters. The first major cluster had only one genotype (BARI begun 6) and the second major cluster had rest of ten genotypes. BARI begun 6 vs BARI begun 1 showed the highest Nei’s genetic distance (0.5261) as they are released from different parental origin. On the other hand, BARI begun 9 vs BARI begun 7 varietal pair showed lowest genetic distance (0.0465) as they are released from same parental origin. The experiment reveals genetic diversity and relatedness among 11 brinjal genotypes which may be informative for the future varietal identification and genetic improvement of this vegetable crop.

Keywords: DNA fingerprinting, Solanum melongena, Genetic diversity
A protein isolation method for western blot to study histones with an internal control protein

A protein isolation method for western blot to study histones with an internal control protein

Authors: Dinh Toi Chu, Yang Tao
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Abstract

Histone modification is one of the attractive targets for epigenetic studies. However, current methods to extract chromatin-associated proteins for western blot of histone modification have some weaknesses such as the loss of housekeeping proteins. In this study, we are presenting a simple method to isolate nuclear protein for studying histone modification by immunoblotting with housekeeping proteins. This method provided high protein concentration from minute tissue samples and importantly, it allowed us to detect acetylated histones together with internal control proteins such as β actin.

Keywords: Protein isolation; histone modification; housekeeping protein
Role of drug metabolic enzymes and transporters in drug-drug interactions between antiretroviral and antituberculosis drugs

Role of drug metabolic enzymes and transporters in drug-drug interactions between antiretroviral and antituberculosis drugs

Authors: Md Masud Parvez, Nazia Kaisar, Jae-Gook Shin
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Abstract

Drug-drug interactions is one of the major determinant in drug development and clinical applications. Individual differences in pharmacokinetics may cause extensive variability in drug efficacy, toxicity and adverse drug reactions, and represent a major concern in drug development. According to world health organization, an estimated 1.2 million patients were diagnosed with HIV/TB coinfection in 2015, and approximately 400,000 coinfected individuals were died in 2016. During treatment of tuberculosis and human immunodeficiency virus (HIV) coinfections, a combination of multiple therapeutic drugs is used, which is potential for interactions between coadministered drugs. Drug metabolizing enzymes and transporters plays vital role in the disposition, pharmacokinetics as well as efficacy and toxicity of many antituberculosis (anti-TB) and antiretroviral (ARVs) drugs. The current understanding of drug-drug interactions involving the first-line antituberculosis and antiretroviral (ARVs) drugs are reviewed and summarized in this article emphasizing the role of drug transporters and metabolic enzymes. It is found that several clinical drug-interactions are caused by the phase I and II metabolic enzymes (cytochrome-P-450s, CYPs, UDP-glucuronosyltransferases, UGTs) and uptake/ efflux transporters, particularly permeability glycoprotein, brest cancer resistance protein, multidrug resistance protein and solute carrier transporters). Overall, this study will be helpful to understand the drug-interactions between the ARV or anti-TB drug regimen or with concomitantly used drugs at a glance. By understanding the unique functions and characteristics of enzymes and transporters, it may use in the management of drug interactions in the treatment of HIV and TB.

Keywords: Drug-drug interactions, Metabolic Enzymes, Membrane Transporters, Antiretroviral and antituberculosis drugs
Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages

Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages

Authors: Akhi Moni
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Abstract

Inflammation is associated with the progression of a variety of diseases. Lipopolysaccharides (LPS) tolerance is recognized to reduce proinflammatory responses. Heme oxygenase1 (HO1) and tristetraprolin (TTP) are induced by LPS tolerance and mediate the antiinflammatory effects. However, it was not clear whether two molecules are linked in LPS tolerance. In this study, we sought to determine whether HO1 associates with TTP to mediate the anti-inflammatory effects of LPS tolerance. LPS treatment significantly increased mRNA and protein level of HO1 and TTP in a time dependant fashion while LPS significantly decreased mRNA and protein level of TNFα in Raw264.7 macrophages. LPS tolerance inhibited TNFα mRNA and protein while HO1 and TTP level was still increased. In HO1 deficient macrophages, LPS tolerance failed to attenuate TNFα mRNA expression but TTP level was still decreased. Our results suggest that HO1 and TTP are functionally linked in mediating anti-inflammatory effects of LPS tolerance. This novel LPS tolerance-HO1-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases.

Keywords: LPS tolerance, anti-inflammatory effects, HO1, TTP
Role of auxin and nitric oxide on growth and development of lateral root of plants: possible involvement of exogenously induced Phot1

Role of auxin and nitric oxide on growth and development of lateral root of plants: possible involvement of exogenously induced Phot1

Authors: Akhi Moni, Mohammad Nazrul Islam, Md Jamal Uddin
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Abstract

Development of root architecture including lateral root formation is a critical event for successful growth of plants. Auxin promotes the production of nitric oxide (NO) in roots, which is required for auxin-mediated root organogenesis. Inhibition of auxin transport reduced phototropin1 (Phot1)-GFP expression at the basal and central part of LRs, indicating role of auxin in Phot1 expression in lateral roots. However, the involvement of auxin-mediated NO on Phot1 expression in LR growth and development yet to be explored. This review gives an insight that auxin-mediated NO may lead to LR growth and development through interacting with Phot1.

Keywords: Auxin, NO, Phot1, LR development
Long term administration of gentamicin affects hemato-biochemical parameters and liver architecture of Swiss Albino mice

Long term administration of gentamicin affects hemato-biochemical parameters and liver architecture of Swiss Albino mice

Authors: Nure Jannat, Tanjina Amin, Nasrin Sultana, Mir Rubayet Jahan, M Rafiqul Islam
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Abstract

Gentamicin is most frequently used aminoglycoside antibiotic. Despite its wide use, the effects of gentamicin have not been clearly studied in relation to alteration of hemato-biochemical parameters and liver injury. In the present study, to evaluate the effects of gentamicin on behavioral, hematological, biochemical and morphological parameters of liver, Swiss albino mice were divided into 4 experimental groups (group A: control; group B: pharmacological dose; group C: pharmacological dose rate with chronic treatment, and group D: high dose with chronic treatment). All the mice from group C and D showed dullness, fearness, roughness of the body coat, anorexia and weakness. Liver weight and size were increased significantly in the mice of group C and D than that of group A or group B. Similarly, in hematological study, Total erythrocyte count (TEC), Total leukocyte count (TLC) and hemoglobin (Hb) % values were decreased significantly, whereas, Alanine amino transferase (ALT) values were increased significantly in the mice of group C and D. In addition, congestion and dark coloration with hepatomegaly were found in treated group C and D. Histological study revealed that the liver parenchyma showed central vein congestion, lymphocytic infiltration, irregular size of hepatocyte and dilatation of sinusoids of treated group C and D. Taken together, our current study suggests that although pharmacological dose of gentamicin has no adverse effect on liver but chronic pharmacological dose or chronic high dose has serious adverse effect on liver. These observations lead us to postulate that gentamicin induces liver tissue damage after long term treatment.

Keywords: Gentamicin, Liver, Morphology, Hepatomegaly, Mice
Morphometry and expression of immunoglobulins-containing plasma cells in the Harderian gland of Birds

Morphometry and expression of immunoglobulins-containing plasma cells in the Harderian gland of Birds

Authors: Mir Rubayet Jahan, Md. Nabiul Islam, Md. Zahirul Islam Khan, Akie Yanai, Koh Shinoda
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Abstract

Johann Jacob Harder first described the Harderian gland in 1694 in deer. It is found in most terrestrial animals and is located within the variable aspects of the orbit. It is believed that this gland is involved in diverse functions. Among these, it has been held to be a site of immune response, a source of thermoregulatory lipids and pheromones, act as photoprotective organ as well as part of a retinal-pineal axis. In birds, this glad was reported first in sparrow in 1918. The Harderian gland is covered by capsule and the connective tissue septa that divide the gland into numerous unequal-sized lobes and lobules. Plasma cells are found in the interacinar space and the lumina of lobules. The recent studies suggest that the Harderian gland act as an immunopotent organ in birds, and that the gland in scavenging birds contains more immunoglobulin-containing plasma cells due to their scavenging nature. Moreover, this gland shows considerable species/strain differences in terms of macro anatomy, microanatomy as well as in the dynamics of immunoglobulin-containing plasma cells among different birds. In this review, these species and strain differences are discussed based on recent studies and several goals of future research are identified.

Keywords: Harderian gland, birds, anatomy, immunohistochemistry, species differences
Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet ‘white’)

Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet ‘white’)

Authors: Sheikh Ashikur Rahman, Md. Shamim Akhter
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Abstract

Seeds of white hyacinth bean available in Khulna, Bangladesh were investigated to assess bioactivities such as antibacterial and cytotoxic properties. For the evaluation of bioactivities solvent extraction was performed by using 50% ethanol and 50% methanol. In the present study, in vitro antibacterial screening was done by the method known as disc diffusion assay. Additionally, the cytotoxic activity was screened by using brine shrimp lethality bioassay. Both the extracts exhibited excellent activity against both gram positive and gram negative bacteria. For ethanolic extract of white hyacinth bean, maximum zone of inhibition was found 17.75 mm at 1000 μg/disc against Staphylococcus epidermidis and 16.75 mm against Staphylococcus aureus. On the other hand, for methanolic extract the maximum zone of inhibition was found as 17.25 mm for 1000 μg/disc against Escherichia coli and 16.75 mm against Pseudomonas aeruginosa. In comparison to ethanolic extract, methanolic extract was found to be more active against all tested microorganisms. Compared to vincristine sulphate (with LC50 of 0.99 μg/ml) both ethanolic and methanolic extracts of hyacinth bean seeds showed toxicity lower than 100 μg/ml and they were 34.67 μg/ml and 45.5 μg/ml, respectively. The LC50 values suggest moderate cytotoxicity of the tested samples. The experimental findings could be correlated with the traditional medicinal uses of the seeds of this plant and showed the rational for further investigation which would be required for isolating the possible bioactive constituents responsible for such activities.

Keywords: Antibacterial activity, brine shrimp lethality bioassay, white hyacinth bean
Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells

Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells

Authors: Mahaboob Vali Shaik , Hymavathi K, Subrahmanyam G
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Abstract

Wharton’s jelly (WJ) is a gelatinous tissue exists in the umbilical cord contains stromal cells, termed as mesenchymal stromal cells (MSCs). Current study designed to characterize the stem cell population both within the cord blood and it’s wharton’s jelly tissue and it’s correlation to neonatal parameters. Both wharton’s jelly and cord blood and their CD34+ and CD90+ expression was enumerated by flow-cytometry. Neonatal parameters along with the mode of delivery were analyzed to correlate with CD34 and CD90 markers. From a total of 20 deliveries, CD34 and CD90 expression pattern showed varied in both samples. Gender and the expression of CD34 and CD90 markers doesn’t show any significant change. Whereas, correlation analysis of weight with CD34+ and CD90+ cell counts showed significant difference and it may have the effect on both cell counts. Neonatal parameters especially birth weight may have the effect on cell count of CD34 and CD90 in both CB and WJ. Current study provide a fast method for mesenchymal cell isolation from Wharton's jelly tissue using the total length of the cord, which may be easily applied along with the UC blood, for the banking of UC.

Keywords: Wharton’s jelly; phenotypic characteristics; therapeutic applications; umbilical cord
Expression patterns of the phosphoproteins and total proteins in TLQP-21 (a VGF derived peptide) treated SH-SY5Y cells

Expression patterns of the phosphoproteins and total proteins in TLQP-21 (a VGF derived peptide) treated SH-SY5Y cells

Authors: Md. Shamim Akhter, Jesús R. Requena
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Abstract

VGF (non-acronymic), belonging to a large granin family, gives rise to a number of bioactive peptides by proteolysis and exert an extensive array of biological effects on energy metabolism, pain modulation, gastric secretion function, reproduction, mood regulation, and, diabetes. Among VGF-derived peptides, TLQP-21 (The first four amino acids, in short TLQP (Thr-Leu-Gln-Pro) generalizes the nomenclature of the peptide by its length) is the most studied although little is known yet about downstream molecular mechanisms of action of VGF-derived peptides like TLQP-21. So here as a preliminary analysis, total protein expression was carried out in addition to the phosphoproteomic study of SH-SY5Y cells treated with TLQP-21, using the same cell extracts. Comparison of simple 1D SDSPAGE gels stained with SYPRO® Ruby protein gel stain was carried out to assess whether changes in protein expression could be seen even at such low separation resolution. Expression of several proteins most likely Microtubule-associated protein 1B (MW 271 kDa), Tubulin beta chain (MW 57), Tubulin beta- 4B chain (MW 50), Alpha-2-macroglobulin (MW 163), etc. in TLQP-21 treated and control samples was found significantly different, indicating that the peptide TLQP-21 exerts biological effects on SH-SY5Y cells. Further studies are required to validate the identity of the modulated proteins, obtained from mass spectrometry. Identification of modulated proteins after TLQP-21 treatment would open new avenues to discover the molecular mechanisms of its physiological and pharmacological state.

Keywords: TLQP-21, SH-SY5Y, VGF, Phosphoprotein, Gel stain
Ocimum herb species: a potential treatment strategy for diabetic kidney disease

Ocimum herb species: a potential treatment strategy for diabetic kidney disease

Authors: Indira Pandiri, Akhi Moni
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Abstract

Ocimum genus most commonly termed as Tulsi, an aromatic plants belongs to family Lamiaceae. In addition to anti bacterial activity, anti-oxidant, anti diabetic, cardio protective, neuroprotective, radio-protective, anti-cancer, and free radical scavenging properties, quite a few studies have reported that this traditionally grown medicinal herb may become a potential therapy for diabetic kidney disease (DKD). This paper will significantly review Tulsi plants role in treatment of DKD to provide reference for future research.

Keywords: Ocimum herb, Medicinal plant, Kidney disease
Molecular characterization and phylogenetic analysis of two minnows, Puntius sarana and Barbodes gonionotus

Molecular characterization and phylogenetic analysis of two minnows, Puntius sarana and Barbodes gonionotus

Authors: Shirin Sultana, Mohammad Shahdat Hossain, Mohammad Nazrul Islam, Md. Shahidul Islam Bhuiyan, Md. Salimullah, Jahangir Alam
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Abstract

Two minnows, indigenous olive barb, Puntius sarana and exotic silver barb, Barbodes gonionotus are important fish species in Bangladesh. Therefore, it is essential to identify diversed population of these fish species for selective breeding programme. Sixty olive barb fish were collected from three different natural stocks (Mymensingh, Madaripur and Sylhet) and 20 silver barbs from hatchery stock of Jashore in Bangladesh. Out of 40 decamer primers tested, 5 primers were selected for the Polymerase Chain Reaction (PCR) based RAPD (Randomly Amplified Polymorphic DNA) analysis. Upon agarose gel electrophoresis, RAPD bands were scored as separately on the basis of their presence or absence for each sample and primer. A total of 43 polymorphic bands and highest proportion of polymorphic bands (62.79%) were found in the Madaripur populations. The gene diversity (0.2132±0.2067) and Shannon's Information index (0.3161±0.2950) within populations were highest in Jashore stock. Among olive barb stocks, these values were higher in Sylhet stock. Besides, four populations segregated in two main clusters based on the Nei’s genetic distance. Indicating the segregation of two different species of minnows, the silver barb stock made one separate cluster while other three stocks of olive barb remained in another cluster. The present study exposed a distinct pattern of genetic variation and phylogenetic relatedness that would be helpful in selecting broodfish for genetic improvement as well as in conservation of these fish species.

Keywords: Genetic diversity, Polymorphic loci, RAPD analysis, Olive barb, Silver barb
Resveratrol attenuates inflammation through tristetraprolin expression in human hepatocytes

Resveratrol attenuates inflammation through tristetraprolin expression in human hepatocytes

Authors: Akhi Moni, Asif Iqbal, Md Jamal Uddin
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Abstract

Resveratrol (Resv), a polyphenolic compound have the anti-inflammatory and antioxidant effects. Tristetraprolin (TTP) destabilizes mRNAs of pro-inflammatory cytokines. However, it was not clear how Resv exerts anti-inflammatory effects on hepatocytes. In this study, we used human hepatoma cell line (HepG2 cells) and analyzed samples using qRT-PCR. Resv increases TTP expression in hepatocytes. Resv attenuates LPS-induced inflammation through TTP expression in hepatocytes. In TTP deficient hepatocytes, LPS failed to attenuate TNFα, iNOS, and IL-6 mRNA expression whereas TTP level was still decreased. Our results suggest that Resv may attenuate inflammation in hepatocytes through TTP induction at least in part. This novel Resv-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases in liver.

Keywords: Resveratrol, TTP, inflammation, hepatocytes
Prophylactic effects of vitamin E and selenium on di (n-butyl) phthalate-induced testicular damage in prepubertal rats

Prophylactic effects of vitamin E and selenium on di (n-butyl) phthalate-induced testicular damage in prepubertal rats

Authors: Mohammad Shah Alam, Md. Nazmul Hoque
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Abstract

Di (n-butyl) phthalate (DBP), a potential endocrine disruptor, adversely affects male reproductive function via activation of oxidative stress. This study was conducted to investigate the protective effects of vitamin E (Vit E) and selenium (Se) on DBP-induced testicular damage. Prepubertal rats were given 500 mg DBP/kg alone and in combination with Vit E (250 mg/kg) & Se (1 mg/kg) by oral gavage for 7 days and sacrificed at day 1 (D1), D30 and D45 after administration. Testicular section of DBP-treated rats showed markedly distorted seminiferous tubules, no spermatids and a reduction in the thickness of their epithelial lining, compared to the control. In contrast, co-administration of Vit E & Se protected the seminiferous tubules and regained its normal architecture to the control level. To clarify whether spermatogenic cells differentiate into mature spermatids in the treated testes at the end of first wave of spermatogenesis, immunostaining for Hsc 70t, a specific marker for spermatids, was carried out. As a result, the increase in maturation of spermatids in Vit E & Se+DBP-treated testes, compared to the DBP-treated, was demonstrated. For example, the most advanced spermatids in the tubules from rats in the DBP-treated groups were steps 8-9 at D45 of recovery, while those of the DBP+Vit E & Se-treated were steps 14-19 that were more or less similar to the control group. These results show for the first time that prepubertal administration of Vit E & Se have protective effects on DBP-induced testicular damage and restoration of normal spermatogenesis.

Keywords: Di (n-butyl) phthalate, Vitamin E, Selenium, Prepubertal rats, Testis

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