3D bioprinting for tissue engineering: Stem cells in hydrogels
Journal Title: International Journal of Bioprinting - Year 2016, Vol 2, Issue 1
Abstract
Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.
Authors and Affiliations
Nazia Mehrban, Gui Zhen Teoh and Martin Anthony Birchall
Keywords
Related Articles
- EP ID EP678638
- DOI -
- Views 184
- Downloads 0
Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation
We report an extrusion-based bioprinting approach, in which stabilization of extruded bioink is achieved through horseradish peroxidase (HRP)-catalyzed cross-linking consuming hydrogen peroxide (H2O2) supplied from HRP a...
A multi-scale porous scaffold fabricated by a combined additive manufacturing and chemical etching process for bone tissue engineering
It is critical to develop a fabrication technology for precisely controlling an interconnected porous structure of scaffolds to mimic the native bone microenvironment. In this work, a novel combined process of additive m...
Bioprinting of osteochondral tissues: A perspective on current gaps and future trends
Osteochondral tissue regeneration has remained a critical challenge in orthopaedic surgery, especially due to complications of arthritic degeneration arising out of mechanical dislocations of joints. The common gold stan...
Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration
Scaffolds are physical substrates for cell attachment, proliferation, and differentiation, ultimately leading to the regeneration of tissues. They must be designed according to specific biomechanical requirements such as...
Of balls, inks and cages: Hybrid biofabrication of 3D tissue analogs
The overarching principle of three-dimensional (3D) bioprinting is the placing of cells or cell clusters in the 3D space to generate a cohesive tissue microarchitecture that comes close to in vivo characteristics. To ach...