A five-year retrospective evaluation of t(4;11) MLL/AF4 translocation results with real time RT-PCR in acute lymphoblastic leukemia patients
Journal Title: Ege Tıp Dergisi - Year 2015, Vol 54, Issue 3
Abstract
Aim: t(4,11) is a chromosomal abnormality formed by the translocation MLL-AF4, which is the result of the fusion of the AF4 gene, localized on 4q21 chromosomal band, to the MLL gene, localized on 11q23 chromosomal band. The aim of this study is to examine the results of the analysis of t (4;11) MLL-AF4 translocation in acute lymphoblastic leukemia (ALL) patients retrospectively. Materials and Methods: Peripheral blood or bone marrow samples of 176 children (70 girls, 106 boys) and 144 adults (60 women, 84 men) with a preliminary diagnosis of acute leukemia between 2009-2013 were analyzed in the Medical Biology Department of Ege University Faculty of Medicine. The translocation RNA results of 71 peripheral blood and 473 bone marrow samples of these patients were evaluated quantitatively for t(4;11) with real-time RTPCR. t(4;11) quantitation was performed by real-time qRT-PCR instrument after the synthesis of complementary DNA with conventional PCR from total RNA or mRNA isolated from blood and bone marrow. Quantitative analysis of the patients was performed by comparing positive and negative controls and samples classified as positive or negative (the ratio of the number of positive copies to the number of reference copies). Results: A total of 320 patients, with 98 having also follow-ups, were evaluated for t(4;11) translocation. Totally 34 patients (24 children and 10 adults) were found positive and the other samples were negative. Conclusion: The assessment of these results supports that, quantitative determination of t(4;11) with RT-PCR method among newly diagnosed ALL patients and ALL patients undergoing treatment, is a valuable method for both confirming the diagnosis and guiding the treatment intended to achieve molecular remission.
Authors and Affiliations
Sunde YILMAZ SÜSLÜER, Burçin TEZCANLI KAYMAZ, Vildan BOZOK ÇETİNTAŞ, Aslı TETİK VARDARLI, Duygu AYGÜNEŞ, Ayşegül DALMIZRAK, Çağdaş AKTAN, Ali Şahin KÜÇÜKASLAN, Tuğçe BALCI, Çağla KAYABAŞ, Besra ÖZMEN YELKEN, Nur SELVİ GÜNEL, Çığır BİRAY AVCI, Buket KOSOVA, Zuhal EROĞLU, Serap AKSOYLAR, Nazan ÇETİNGÜL, Can BALKAN, Deniz YILMAZ, Yeşim AYDINOK, Kaan KAVAKLI, Mahmut TÖBÜ, Murat TOMBULOĞLU, Filiz BÜYÜKKEÇECİ, Fahri ŞAHİN, Güray SAYDAM, Cumhur GÜNDÜZ
Keywords
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- EP ID EP342592
- DOI 10.19161/etd.344127
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