A New Approach for the Treatment of Diabetes and Nicotine Induced Cataract by Modulating Specific Ion-Channels Function in Rat Lens
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 5, Issue 4
Abstract
Aim: Cataract is an eye disease characterized by a cloudiness of the normally transparent crystalline lens. Diabetes and smoking are the known risk factors for cataract development. It is well known fact that inorganic ions like Na+, K+, Ca++ and Cl- play an important role for the maintenance of lens hydration and transmittance. Nicotine aggravates cataract formation in diabetic patients by disturbing the ionic balance in the lens, generation of free radicals and disturbing normal lens physiology by generation of free radicals. Based upon these observations we have screened few drugs like Lidocaine, Nifedipine and Phenylglycine against Streptozotocin (STZ) + Nicotine induced cataract. Methodology: Diabetes was induced by administration of combination of STZ (single dose of 52 mg/kg i.p.) and nicotine (0.3 mg/kg s.c) for 22 consecutive days and simultaneously treated with ophthalmic preparation of test drug i.e. Lidocaine, Nifedipine and Phenylglycine at 1% and 2% to the right and left eye respectively. On 23rd day of the study, various parameters like measurement of various ions (Na+, K+, Ca++ and Cl-), anti-oxidants (ascorbic acid, sulfhydral group, glutathione) and fructose content in rat lens were studied. We have also investigated the level of ascorbic acid in serum and monitored the blood glucose level at regular interval throughout the experiment. Results: Accumulation of fructose in the lens cause malfunctioning of Na+-K+- ATPase pump which leads to accumulation of Na+ ions inside the membrane that causes to accretion of water and osmotic swelling of the lens. By the treatment with lidocaine (1% and 2%) eye drop significantly (P<0.001) reduced the Na+ content in both the eyes compared to disease control group. Conclusion: In our study lidocaine has offered best protection against cataract. Phenylglycine has also shown protection but not as good as lidocaine, at the same time Nifedipine has not shown any protection.
Authors and Affiliations
Vijay R. Chidrawar, Krishnachaitanya Gudlawar, Sushma Mondi, Uma Maheshwararao Vattikutti
Keywords
Related Articles
- EP ID EP344748
- DOI 10.9734/BJPR/2015/14002
- Views 98
- Downloads 0
Antifilarial Activity of Cucurbita pepo ovifera var ovifera (Cucurbitaceae) on Onchocerca ochengi Adult Worms
One of the strategies for developing novel pharmaceutical drugs is to use natural sources such as plants for therapeutic treatment. Plant extracts are a cocktail of compounds which act synergically and can improve treatm...
New Perspectives on Asphodeline lutea from Bulgaria and Turkey: Anti-mutagenic, Anti-microbial and Anti-methicillin Resistant Staphylococcus aureus (MRSA) Activity
Aims: In this study, methanol extracts of Asphodeline lutea roots from Bulgaria (ALB) and Turkish (ALT) origin were evaluated for their anti-microbial, anti-MRSA properties and they were also screened for the potential o...
Assessment of the Hematological Parameters of Albino Rats Fed on High Dose of whole Wheat
Aim: The aim of this study was to investigate the hematological profile of albino rats fed on whole wheat based on its widespread use. Whole wheat grain (Triticum aestivum) is a cereal regularly consumed and also used fo...
Isolation, Characterization and Antimicrobial Activity of 3β, 22E-Stigmasta-5, 22-dien-3-ol from the Aerial Part of Aeschynomene uniflora E. Mey
Objectives: To isolate, characterize and determine the antimicrobial activity of bioactive compounds present in the petroleum ether fraction of the aerial parts of Aeschynomene uniflora E. Mey. Methods: Aeschynomene unif...
A Comparison of the Humoral Immune Response Induced by a Recombinant Human Protein in Wild Type Mice and in Transgenic Mice Expressing the Protein
Aim: The aim of this work was to investigate the correlation between anti drug antibody (ADA) induction and how different manufacturing processes of biopharmaceuticals affect the immunogenicity of the protein. This was d...