A non-traditional therapeutic strategy in management of fulminant hepatic failure: histopathological and immunohistochemistry study
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2014, Vol 2, Issue 1
Abstract
The present study was undertaken to evaluate the crucial role of hepatocytes transplantation in experimental model of D-galactosamine induced fulminating hepatic failure (FHF) in rat. Hepatocytes were isolated and grown from a group of apparently healthy rats. The isolated hepatocyte were characterized and cultured in vitro and transplanted in FHF rat models either alone or with cyclosporine as immunosuppressive agent. The isolated hepatocytes were cultured for 24 hours prior transplantation in the peritoneal cavity in each of infected (FHF) rat. It was observed that the high survival rate and the improvement of the liver function parameters among transplanted groups versus the non-treated ones. Histopathological and immunehistochemistry data revealed the presence of hepatocytes attached to the peritoneal covering of the abdominal organs in either a single or several layers.
Authors and Affiliations
Farid A. Badria*| Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT, Atallah F. Ahmed| Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT, Wael M. Elsaed| Department of Anatomy and Embryology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT, Omar M. Gabre| Department of Anatomy and Embryology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT, Kamal G. Botros| Department of Anatomy and Embryology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT
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