A novel ordered arrangement of composite magnetic nanoparticle polycaprolactone fibrous nerve conduits for peripheral nerve injury repair
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 6
Abstract
Objective To explore the effect of a novel ordered arrangement of composite magnetic nanoparticle polycaprolactone (PCL) fibrous nerve conduits for peripheral nerve injury repair. Methods The PCL nerve conduits with composite Fe3O4 nanoparticles were prepared by electrospinning technique to comparatively analyze the role of Fe3O4 nanoparticles in nerve injury repair. Nerve fibrous conduits with or without Fe3O4 nanoparticles were first prepared, and the mechanical properties of the two kinds of nerve conduits were analyzed by scanning electron microscopy and atomic force microscopy. Twenty-eight male SD rats were randomly divided into blank control group, simple disordered PCL nerve conduit group, simple ordered PCL nerve conduit group and compound magnetic nanoparticles ordered PCL nerve conduit group, with 7 rats in each group, and they were operated on 12-mm sciatic nerve defects, respectively. No special treatment was given to blank control group, and nerve conduits in different conduit groups were sutured. Nerve repair ability was observed by HE staining, and the functional recovery was analyzed by sciatic functional index and gastrocnemius staining. Results The results of atomic force microscopy showed that the surface of the nerve conduits containing Fe3O4 nanoparticles was rougher and more conducive to the adhesion and growth of nerve axons. The results of scanning electron microscopy showed that the orderly structure of PCL fibrous nerve conduits was obviously observed, which provided a suitable growth environment for the directional growth of nerves. In the in vivo nerve repair experiment, the functional scores and histological repair effects of the ordered nerve conduit group combined with composite Fe3O4 nanoparticles were significantly better than those of the blank control group (P < 0. 01), simple disordered PCL nerve conduit group (P < 0. 01) and simple ordered PCL nerve conduit group ( P < 0. 05). Conclusion The ordered PCL fibers with magnetic nanoparticles can effectively promote the orderly growth of nerve axons and accelerate nerve injury repair.
Authors and Affiliations
LIU Xingchen, GAO Xue, ZHANG Di, LI Shengyou, GAO Jianbo, GUO Lingli, LUO Zhuojing, HUANG Jinghui, XIA Bing
Keywords
Related Articles
- EP ID EP727741
- DOI -
- Views 50
- Downloads 0
Research progress on in vitro models of pulmonary fibrosis
In recent years, the incidence of pulmonary fibrosis is increasing year by year, and 10% of patients with coronavirus disease 2019 will also develop pulmonary fibrosis injury-like lesions. However, there is still no maj...
Analysis of risk factors for surgical site infection after colon cancer surgery in the elderly
Objective To analyze the medical records of postoperative surgical site infection ( SSI) in elderly patients with colon cancer after radical resection of colon cancer, and to explore the risk factors for the occurrence...
Research progress of the role of NAD + in sulfur mustard poisoning
Sulfur mustard (SM), as a classic erosive chemical agent, has caused a large number of casualties and is still a potential threat. SM can cause damage to multiple organs and systems such as skin, eyes, and respiratory s...
Correlation between immune cell infiltration and patient outcomes in renal cell carcinoma
Objective To use bioinformatics methods to analyze the relationship between different degrees of immune cell infiltration in tumors and prognosis of renal cell carcinoma (RCC), so as to provide a theoretical basis for c...
Correlation between refractive status and ocular biological parameters among students of grade three in teenagers aviation schools of Air Force
Objective To analyze the correlation between refractive status and ocular biological parameters among students of grade three in teenagers aviation schools of Air Force, and to explore the key monitoring indicators for...