A Study of Bio-Film Production and Antibiotic Sensitivity Pattern of Proteus Species Isolated From Pus and Urine Samples
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 11
Abstract
Abstract: Proteus species is a member of the Enterobacterieceae family. It is identified as one of the etiological agents of hospital acquired infections. The expanding profile of antibiotic resistance among Proteus species is a clinical threat. It is also one among the biofilm forming organisms. The present study was undertaken to determine biofilm production in Proteus species by three different phenotypic methods and to detect Extended spectrum betalactamase and Amp C betalactamase production among the isolates and to know the distribution of resistant strains in biofilm producing and non-producing isolates. A total of 100 proteus isolates from different samples were screened for biofilm production by Congo red agar (CRA) method, Tube method (TM) and Tissue culture plate (TCP) method. Antibiotic susceptibility was determined by Kirby Baeur’s disc diffusion method following CLSI guidelines. ESBL and AmpC betalactamase production was detected by disc potentiation tests. Out of 100 Proteus isolates, 71 were P.mirabilis and 29 were P. vulgaris. Among them 69 were biofilm producers. ESBL production was detected in 62% and AmpC betalactamase in 44% of isolates and 28% were multidrug resistant strains. Resistance is more noticed in biofilm producers than non-producers. As there is increasing pathogenicity of Proteus species, they should be identified to species level and resistance pattern to be screened by simple, reliable and preferably inexpensive methods.
Authors and Affiliations
Praveen Kavuri, Rajyalakshmi Gunti, Ratna Kumari Gorinka
Keywords
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