A Study of Immune Dysfunction in Patients with Breast Carcinoma
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2016, Vol 18, Issue 2
Abstract
Immune system play a major role in malignant transformation and to understand the role of immune cells in breast carcinoma. We have evaluated lymphocytes, monocytes, neutrophils, ALC (absolute lymphocyte count), AMC (absolute monocyte count), ANC (absolute neutrophil count), ATC (absolute T-cell count), AHTC (absolute helper T-cell count), and ACTC (absolute cytotoxic T-cell count) expression. The flowcytometry method applied for evaluation of CD markers for r e s p e c t i v e i m m u n e c e l l s i n p e r i p h e r a l b l o o d , a n d immunohistochemistry methodology applied for T-cell and neutrophils infiltration in tumor tissue of breast carcinoma patients. The median value of neutrophils (p = 0.04) and absolute count of neutrophils was found higher, while median value of lymphocytes was lower in breast carcinoma patients than healthy controls. However no difference in median value of monocytes, absolute lymphocyte counts (ALC), absolute monocyte count (AMC) was noted between these two groups. Further, median value of CD3+ T-cells and CD8+ cytotoxic T-cells was found lower and CD4+ Helper T-cells was found higher in breast carcinoma patients as compared to healthy women. In relation to clinico-pathological parameters, the median value of CD4+ helper T-cells was found significantly lower in grade III and median value of CD8+ cytotoxic T-cells was found significantly increased in BR Score 8 tumors. In tumor microenvironment, CD3+ T-cell infiltration were seen in 98% of patients. A significant higher incidence of CD3+ T-cell infiltration (2+ or 3+ score) was seen in patients with >48 years and BR Score 8 tumors. Further, patients with CD3+ T-cells infiltration in tumor microenvironment with 2+ or 3+ score had low median level of helper T-cells (CD4+) in circulation as compared to patients with 0 or 1+ score. This study observed immune suppression in breast cancer patients and antitumor activity of CD3+ T-cells in tumor microenvironment.
Authors and Affiliations
Atiya Saiyed, Birva Raiya, Hemangini Vora
Keywords
Related Articles
- EP ID EP197194
- DOI -
- Views 54
- Downloads 0
Cytoplasmic Her-2/neu Internal Domain Expression a Truncated form Confirmed by Double Staining Immunohistochemistry Identifies an Aggressive Breast Cancer Phenotype
Her-2/neu over expression is found in women with breast cancers and recent studies indicated prognostic significance of Her-2/neu over expression may be due to associated expression of p95Her-2/neu, a truncated form of H...
Summaries of Presentations at Clinical Meetings
Salivary gland tumors often pose a diagnostic challenge since they can show a striking range of morphological diversity, benign tumors have a propensity for malignant change and special stains & IHC have limited role. Ca...
Dr. T. B. Patel Oration Award Year - 2015
Cervical Cancer Control: The Dawn of a New Era
Spirituality and Attitude of the Medical Profession
How often are you faced with a situation where your patient equates you with God while thanking you. In India this situation is not rare. It is an embarrassing situation for most, irritating for some due to the over exag...
Epithelial Mesenchymal Transition Markers in Breast Cancer
Epithelial-mesenchymal transition (EMT) is defined by the loss of epithelial characteristics and the acquisition of a mesenchymal phenotype. It can be associated with increased aggressiveness, and invasive and metastatic...