A study of serum iron level and oxidative stress in the steady state of sickle cell of anaemia
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2017, Vol 4, Issue 1
Abstract
Background: Sickle cell anaemia (SCA) is a hereditary disorder, associated with severe haemolytic anaemia, periodical vasooclussive pain, and premature death. Prevalence of SCA is alarmingly high in north Maharashtra comprising districts of Nandurbar, Dhule, Jalgaon and Nashik. Non heme iron and depleted erythrocytic glutathione are associated with generation of free radicals leading to oxidative stress. Oxidative stress may enhance haemolysis by damaging erythrocytic membrane. Aim and Objectives: To evaluate serum iron, erythrocytic MDA and erythrocytic Glutathione in relation with possible markers of oxidative stress in the steady state of SCA individuals. Methods: A total of 90 subjects were recruited in the study including 30 sickle cell trait (HbAS), 30 sickle cell disease (HbSS) and 30 healthy controls. In all subjects serum iron and bilirubin, erythrocytic Malondialdehyde (MDA) and glutathione (GSH) concentration were investigated simultaneously. Results: Serum iron level found significantly (p<0.001) lowered in sickle cell trait (HbAS) while significantly (p<0.001) elevated in sickle cell disease (HbSS) compared to control. Serum bilirubin and Erythrocytic MDA found significantly (p<0.001) elevated in HbAS and HbSS. Erythrocytic glutathione was found significantly (p<0.001) depleted in both HbAS and HbSS in comparison of control. There is positive correlation between erythrocytic MDA and serum iron. Conclusion: Our study suggests, elevated serum iron and bilirubin is result of excessive hemolysis. Depleted Erythrocytic glutathione and elevated malondialdehyde reveals hyphened oxidative stress, further which could be sickling and hemolytic propagator aspect in sickle cell anaemia.
Authors and Affiliations
Prakash S. Hundekar, Adinath Suryakar, Aarti Karnik, Rahul Ghone, Sonali Bhagat
Keywords
Related Articles
- EP ID EP295857
- DOI 10.18231/2394-6377.2017.0013
- Views 62
- Downloads 0
A Study of Serum Magnesium, Calcium and Phosphorus in Hypothyroidism
Introduction: Hypothyroidism, the commonest form of hormonal dysfunction, is due to thyroid hormone deficiency or its impaired activity. Various electrolyte disturbances in association with hypothyroidism were observed i...
A Multi Marker Evaluation of Cardiovascular Disease Risk in Patients with Rheumatoid Arthritis
Background: Rheumatoid arthritis (RA), an inflammatory joint disease, is also associated with systemic effects. Patients with RA have an increased risk of morbidity and mortality that is mainly attributable to cardiovasc...
Qualitative chemical analysis and incidence of renal stone disease in rural population of South Solapur, Maharashtra
Background: There are many reports regarding renal stone disease, which have documented that it is a complex disease process and widely distributed in developed as well as developing world with varied chemical constituti...
Role of free radicals and antioxidants in cervical cancer in southern part of Rajasthan
Introduction Present study is conducted to measure the role of free radicals malondialdehyde and vitaminC and vitaminE in cervical cancer women in southern part of RajasthanMaterials and Methods Total 80 cases and contro...
Evaluation of hsCRP, Insulin and Insulin resistance in Polycystic Ovarian Syndrome
Introduction: Polycystic Ovarian Syndrome consists of a constellation of metabolic abnormalities and is now well recognised having a major effect throughout life on the reproductive, metabolic and cardiovascular health o...