A Study on Biofilm Production and Antibiotic Susceptibility Profile of Clinical Staphylococcus aureus Isolates in a Tertiary Care Hospital
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 12
Abstract
Abstract: Staphylococcus aureus is one of the most important human pathogens and has been a leading cause of hospital and community acquired infections. Bacteria protected by biofilms are resistant to host defense mechanisms and show resistance to standard antibiotic therapy.This study was conducted to determine the ability of Staphylococcus aureus to produce biofilm and to determine the antibiotic susceptibility profile of Staphylococcus aureus isolated from various clinical samples. A total of 100 non-repetitive strains of Staphylococcus aureus isolated from various clinical samples from Jan2018 - June2018 were included in the study. All the isolates were identified using standard microbiological procedures. Antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion technique following Clinical and Laboratory Standards Institute (CLSI) guidelines. All these staphylococci were tested for biofilm production by tube method. Among 100 Staphylococcus aureus isolated from various clinical samples, 42 (42%) were biofilm positive and 58 (58%) were biofilm negative. Among 42 biofilm positive Staphylococcus aureus, 5 were from urine samples, 25 were from pus samples and 12 were from sputum samples. The resistance to penicillin,ciprofloxacin, chloramphenicol and oxacillin was significantly more( p<0.05) in biofilm producing Staphylococcus aureus. All the staphylococcal isolates were sensitive to Vancomycin and Linezolid (100%). Our study showed that 42% of clinical staphylococcus aureus isolates were biofilm producers. Biofilm producing isolates showed higher resistance to all the antibiotics than biofilm non-producers. Thus all the clinical isolates of Staphylococcus aureus should be routinely screened for biofilm production.
Authors and Affiliations
Manoj P, Dr. Mamatha P Samaga
Keywords
Related Articles
- EP ID EP480815
- DOI -
- Views 55
- Downloads 0
New Treatment Methods to Treat Tuberculosis
Abstract: Internationally it is estimated that around 273,000 new cases of multidrug-resistant tuberculosis (MDR-TB, resistant to isoniazid and rifampicin) occurs every year. To improvise and efiiciently manage MDR-TB i...
An easy and economical method of construction of tissue microarray
Abstract: Tissue microarray is very useful in research. However the automated equipment is very costly and cannot be afforded by many laboratories. We describe a very simple and cheap manual method of preparation of tiss...
A Study of Anatomical Variations in the Caeco-Appendicular Position
The caecum and appendix are derivatives of the midgut, situated in the right iliac fossa. The caecum is a capacious sac like segment of the proximal colon. The terminal ileum empties in to the caecum through the ileo–cae...
Correlation of Classroom Attendance with Exam Performance in Ist MBBS Students In South Maharashtra
Abstract: A number of studies have shown the impact of attending the classes during the session on examination results of students. Students with good lecture attendance show good results while those with poor lecture at...
Analysis of Sociodemographic Characteristics as Risk Factors for Cesarean Delivery in Induced Labor at Term
Abstract: To analyse sociodemographic characteristics as risk factors for cesarean delivery in induced labor at term. Descriptive type of Observational study at Department of Obs & Gynae Department, S.M.S. Medical Colleg...