A Study To Find Out Diagnostic Accuracy of Calcoflour White Stain Among Patients (Mycotic Keratitis) Attending Ophthalmology Department of SMS Hospital Jaipur
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2017, Vol 2, Issue 3
Abstract
Background: Fungal keratitis is a suppurative, ulcerative and sight threatening infection of the cornea that some time leads to loss of the eye. Corneal infection of fungal etiology is very common. Methods: The present study has been carried out on 100 patients of confirmed keratitis or suspected keratitis in the department of Microbiology and department of Ophthalmology, SMS Hospital, Jaipur (Rajasthan) from January 2015 to December 2016. Results: In this study, positive fungal culture was taken as the gold standard showing a sensitivity of KOH+CFW for diagnosing mycotic keratitis was 69.23% and specificity was 96.82%.The PPV for CFW was 85.18% and NPV was 78.08%.The sensitivity of KOH was 58.97% and specificity was 93.44%.PPV for KOH was 93.1% and NPV was 78.08%. Conclusion: Calcofluor white staining was found to be more sensitive as compared to KOH wet mount smear in demonstrating fungi in direct microscopy. In addition, Calcofluor white stain decreased the chances of false negative results. Calcofluor white stain is highly sensitive method for detection of fungi in mycotic keratitis and should be performed routinely before declaring the corneal scrapings negative for fungi.
Authors and Affiliations
Dr. Shilpa Balodia
Keywords
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