A validated analytical method for the estimation of Oxetacaine from its pharmaceutical formulation by RP-HPLC
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 5
Abstract
OBJECTIVE: The aim of the current study was to develop an analytical procedure for the determination and quantification of Oxetacaine, from its marketed formulation which is simple, precise, accurate and a validated, reverse phase high performance liquid chromatography (RP-HPLC) method. METHOD: The optimized chromatographic condition was achieved on a kromasil C18 (150 X 4.6 mm i.d., 5µ) as stationary phase and Acetonitrile: 10mM Potassium dihydrogen-ortho-phosphate (pH 3.5) in the ratio 30:70 % v/v as mobile phase, with a flow rate of 1.0 ml/min. The detector response for the method was determined, and the quantification was carried out at 214 nm. RESULT: Oxetacaine was eluted at 6.0 min. The quantification was performed using calibration curve method, and the linearity was achieved from 10-50 µg/ml. The percentage recovery was found to be 98.0 ± 1.20 respectively. The correlation coefficient was found to be 0.9954 respectively. The limit of detection (LOD) and limit of quantification (LOQ) for the current method was achieved at 1µg/mL and 5µg/mL respectively. CONCLUSION: The current study was performed at ambient temperature, and the method is simple, selective, linear, precise, accurate and sensitive which can be used for the routine analysis of Oxetacaine tablets. The developed method was validated as per ICH guidelines.
Authors and Affiliations
S. T. Narenderan| Department of Pharmaceutical Analysis, B. Babu| Department of Pharmaceutical Analysis, S. N. Meyyanthan| Department of Pharmaceutical Analysis, B. Gowramma| Department of Pharmaceutical Chemistry,JSS College of Pharmacy (A Constituent College of Jagadguru Sri Shivarathreeswara University, Mysore), Udhagamandalam, India
Keywords
Related Articles
- EP ID EP16037
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(5).2764-2
- Views 417
- Downloads 18
Optimization of Diesel oil degrading Bacterial strains at various culture parameters
Environmental pollution with petroleum has been recognized as one of the most serious current problems. Petroleum hydrocarbons are a combination of hydrocarbons obtained from reservoirs of crude petroleum. The petroleu...
ISOLATION, PURIFICATION AND CHARACTERIZATION OF ALKALI AND THERMO STABLE XYLANASE FROM BACILLUS SP. KS09
Nine bacterial strains were isolated using xylan rich media. The bacterial strain KS09 was selected on the basis of qualitative and quantitative test. It was identified as Bacillus sp. via physiological, morphological a...
EUDRAGIT-BASED NANOSTRUCTURES: A POTENTIAL APPROACH FOR OCULAR DRUG DELIVERY
Drug delivery in ocular therapeutics is a challenging problem and is a subject of interest to scientists working in the multidisciplinary areas pertaining to the eye. Most ocular diseases are treated by topical drug app...
Preparation and in vitro and in vivocharacterization of Solid Dispersions tablets of Azithromycin by melting method
Azithromycin is a cyclic-structure macrolide, shows prolonged antibacterial, anti-inflammatory and immunomodulatory effects. Azithromycin belongs to BCS class II drug i.e. drug with poor solubility and good permeability...
COMPARATIVE IMMUNOMODULATOR ACTIVITY OF LEAVES AND BARK OF ALBIZIA LEBBECK (LINN.) BENTH
Adaptability is probably the most distinct characteristics of life which may be defined as sum of all non specific response of the body to any demands made upon it; fundamentally it was a physiological response; primary...