A validated analytical method for the estimation of Oxetacaine from its pharmaceutical formulation by RP-HPLC
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 5
Abstract
OBJECTIVE: The aim of the current study was to develop an analytical procedure for the determination and quantification of Oxetacaine, from its marketed formulation which is simple, precise, accurate and a validated, reverse phase high performance liquid chromatography (RP-HPLC) method. METHOD: The optimized chromatographic condition was achieved on a kromasil C18 (150 X 4.6 mm i.d., 5µ) as stationary phase and Acetonitrile: 10mM Potassium dihydrogen-ortho-phosphate (pH 3.5) in the ratio 30:70 % v/v as mobile phase, with a flow rate of 1.0 ml/min. The detector response for the method was determined, and the quantification was carried out at 214 nm. RESULT: Oxetacaine was eluted at 6.0 min. The quantification was performed using calibration curve method, and the linearity was achieved from 10-50 µg/ml. The percentage recovery was found to be 98.0 ± 1.20 respectively. The correlation coefficient was found to be 0.9954 respectively. The limit of detection (LOD) and limit of quantification (LOQ) for the current method was achieved at 1µg/mL and 5µg/mL respectively. CONCLUSION: The current study was performed at ambient temperature, and the method is simple, selective, linear, precise, accurate and sensitive which can be used for the routine analysis of Oxetacaine tablets. The developed method was validated as per ICH guidelines.
Authors and Affiliations
S. T. Narenderan| Department of Pharmaceutical Analysis, B. Babu| Department of Pharmaceutical Analysis, S. N. Meyyanthan| Department of Pharmaceutical Analysis, B. Gowramma| Department of Pharmaceutical Chemistry,JSS College of Pharmacy (A Constituent College of Jagadguru Sri Shivarathreeswara University, Mysore), Udhagamandalam, India
Keywords
Related Articles
- EP ID EP16037
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(5).2764-2
- Views 364
- Downloads 18
SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF SOME NEWER BIPHENYL IMIDAZO [2,1-b] [1,3,4]THIADIAZOLE DERIVATIVES
In the present study, we have reported the synthesis of some novel heterocyclic derivatives comprising imidazole and 1,3,4-thiadiazole containing moiety. Imidazothiadiazoles are of interest because of their diverse biol...
CHARACTERIZATION OF HIGH ACTIVITY DRUG-STIMULATED ATPase OF MULTIDRUG RESISTANT LEISHMANIA DONOVANI AMASTIGOTE CELLS
Background: The emergence of drug resistance and the limited knowledge of the mechanisms by which the parasites acquire resistance are the major obstracle for the control of leishmaniasis. Methods: The present investig...
ISOLATION, PURIFICATION AND CHARACTERIZATION OF ALKALI AND THERMO STABLE XYLANASE FROM BACILLUS SP. KS09
Nine bacterial strains were isolated using xylan rich media. The bacterial strain KS09 was selected on the basis of qualitative and quantitative test. It was identified as Bacillus sp. via physiological, morphological a...
A review on Mucoadhesive Buccal Patches
The oral cavity is an attractive site for the delivery of drugs. Through this route it is possible to realize mucosal (local effect) and transmucosal (systemic effect) drug administration. In the first case, the aim is...
ANTIULCER ACTIVITY OF PETROLEUM ETHER EXTRACT OF BETA VULGARIS (L.)
Objectives: The present study was designed to evaluate the antiulcer activity of petroleum ether extract of Beta vulgaris L. Methods: Present study was designed to investigate the antiulcer activity of petroleum ether e...