A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2013, Vol 7, Issue 2
Abstract
The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substances in veterinary formulation. This formulation was submitted to accelerated degradation studies under acidic, alkaline and oxidative conditions, exposure to light and thermal stability. The separation of furazolidone, oxytetracycline and degradation products was achieved on BDS Hypersil C18 (250mmx4.6mm, i.d. 5 µm particle size) with gradient mobile phase containing methanol and 80 mM dipotassium phosphate pH 7,5 (20/80). The flow rate was 1.0 mL/min and detection was set at 254 nm, at 25 0C. The developed method was validated with respect to linearity, limits of detection and quantification, specificity, accuracy, and precision.
Authors and Affiliations
Violeta Giugiu
Keywords
Related Articles
- EP ID EP157149
- DOI -
- Views 123
- Downloads 0
Instructions for Authors
Elements of bacterial structure and mechanisms of antibiotic resistance transmission
The aim of this bibliographic essay is to refresh the knowledge of veterinarians in the field of therapy and bacterial resistance. Are summarized, in a didactic manner: the bacteria classification, the overall structure...
Despre evoluția și implicațiile fenomenului rezistenței la medicamentele antiinfecțioase și antiparazitare de uz veterinar și evoluția acestui fenomen în România
This report is based on scientific studies from the last decade, books, experts’ oppinions and based on personal experience. Of course, as part of a process of continuous updating, this view must be regularly adjusted to...
Algorithm of calculation of predicted concentration (PEC) for evaluation of the environmental risk of veterinary medicinal products
The environmental risk analysis for veterinary medicinal products is an assessment of their possible evolution, exposures and effects and is structured according to the VICH GL6 (Phase I) and GL38 (Phase II) guides. The...
Forced degradation of metronidazole, oxytetracycline and furazolidone studies using High Performance Liquid Chromatography
The study describes the development of a HPLC -DAD method for the estimation of oxytetracycline and furazolidone in Enteroguard M - powder. The proposed method utilizes a Betasil C 18 column, (4.6 x 250 mm, 5 µm), at 300...