ACTIVITY AND KINETIC PROPERTIES OF SUPEROXIDE DISMUTASE OF THE SULFATE-REDUCING BACTERIA DESULFOVIBRIO PIGER VIB -7 AND DESULFOMICROBIUM SP. ROD-9
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 4
Abstract
The aim of our work was to study superoxide dismutase activity of cell-free extracts of Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine and to carry out the analysis of the enzyme reaction kinetic properties. Methods. Microbiological, biochemical, biophysical methods, and methods of statistical processing of the results were used. Results. Specific activity of superoxide dismutase from the sulfate-reducing bacteria was studied. The influence of different temperature, pH and substrate concentration on the activity of studied enzymes was showed. The analysis of the kinetic properties of the superoxide dismutase in the bacterial strains, based on experimental data, was carried out. The initial (instantaneous) reaction rate (V0), maximum rate of the enzyme reaction (Vmax), and Michaelis constants (Km) of the superoxide dismutase reaction for both strains was calculated. Conclusion. The kinetic parameters of superoxide dismutase reaction in both bacterial strains were not statistically different. The obtained data on optimum of pH (7.5–9.5) on the activity of the enzyme were consistent to the environment conditions of the human large intestine. These conditions can provide the intensive growth of the bacterial strains, process of the dissimilatory sulfate reduction and, thus, the accumulation of hydrogen sulfide in the gut.
Authors and Affiliations
I. V. Kushkevych, H. L. Antonyak, R. V. Fafula
Keywords
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- EP ID EP395692
- DOI 10.18524/2307-4663.2014.4(28).48409
- Views 90
- Downloads 0
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