Acute and chronic toxicity studies of hydromethanol leaf extract of Helianthus annuus Linn. in rats
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 9
Abstract
Objective: To investigate the safety levels of hydromethanol leaf extract of Helianthus annuus Linn. (H. annuus) in rat. Methods: Acute oral toxicity test of hydromethanol leaf extract of H. annuus was conducted through up and down method at 2.00 g/kg dose limit in rats. The chronic toxicity study was conducted by administering different concentrations (0.25, 0.50 and 1.00 g/ kg) of hydromethanol extract of H. annuus in feed, for 90 consecutive days. On days 30, 60 and 90, blood samples were collected from the retro-orbital plexus of the rats for determination of serum biochemical parameters. Histopathological examination of the pancreas, livers, kidneys and testis were also conducted. Results: The LD50 of the hydromethanol extract of H. annuus was greater than 2.00 g/kg and it significantly (P < 0.05) reduced serum cholesterol. On days 60 and 90, the serum urea and creatinine levels of hydromethanol extract of H. annuus treated groups were elevated when compared with the control group. There were fibrosis in the kidneys and livers; degeneration and necrosis in the testis and significant dose-dependent increases in number and size of pancreatic islet of langerhans. Conclusions: The findings suggest that hydromethanol extract of H. annuus is tolerated in short term administration, but long term (up to 90 days) administration at high doses, may elicit hepatic, testicular and nephrotic disorder.
Authors and Affiliations
Samuel O Onoja
Keywords
Related Articles
- EP ID EP395331
- DOI 10.4103/1995-7645.242311
- Views 89
- Downloads 0
A study on the stable inheritance of chromosome aberration karyotype in three families of two generations in Hainan province
Objective: To examine chromosomes of a total of 10 857 people who asked for prenatal genetic counseling in our hospital from February 1994 to July 2018. The important diagnostic reference index for the clinical diagnosis...
Viral metagenomics analysis of poultry faeces in live poultry market, Haikou, China
Objective: To conduct in-depth study of the distribution and diversity of viruses in poultry is of great importance in monitoring the emergence of interspecies transmission of novel viruses that may cause epidemics with...
Comparative molecular docking studies of lupeol and lupenone isolated from Pueraria lobata that inhibits BACE1: Probable remedies for Alzheimer's disease
Objective: To discover lead lupane triterpenoid's potential isolated from Pueraria lobata roots against b-site amyloid precursor protein cleaving enzyme 1 (BACE1), which serve as a rate limiting step in amyloid beta (Ab)...
Medical plant extracts and natural compounds with a hepatoprotective effect against damage caused by antitubercular drugs: A review
Drug-induced liver injury encompasses a spectrum of diseases ranging from mild biochemical abnormalities to acute liver failure; example of this scenery is hepatotoxicity caused by the first-line antituberculous drugs is...
Ultrasound-assisted extraction of antioxidant polyphenolic compounds from Nephelium lappaceum L. (Mexican variety) husk
Objective: To reach the recovery and identification of antioxidant polyphenolic compounds from Nephelium lappaceum L. (Mexican variety) husk using ultrasound-assisted extraction and liquid chromatography/mass spectrometr...