Adenovirus-mediated overexpression of gamma interferon in murine bone marrow-derived dendritic cells affects their viability and activity
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 0
Abstract
Objective: To explore the effects of induction of Gamma-interferon(IFN-γ) production by adenoviral vectors (AdVs) on dendritic cells (DCs) viability and activity. Methods: AdVs were propagated in human embryonic kidney 293 cell lines and DCs were derived from mouse bone marrow using GM-CSF-IL-4-enriched media. The DCs were infected by either AdVs-green fluorescent protein or AdVs-IFN-γ during a 48 h culture. Phenotypic and functional characteristics of AdV-infected DCs were measured by flowcytometry-based methods. Results: Our results displayed that AdVs-IFN-γ could significantly induce DCs necrosis. Furthermore, AdVs-IFN-γ-infected DCs efficiently expressed the CD86 molecules. Conclusions: According to our finding, AdV-IFN-γ is able to affect murine bone marrowderived DC viability and activity.
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- EP ID EP230475
- DOI 10.1016/S2222-1808(14)60470-9
- Views 55
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