Agar Gel Alkaline Electrophoresis As A Cost Effective Screening Modality For Abnormal Hemoglobins
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 4
Abstract
Alkaline agar gel electrophoresis is a basic manual laborious technique for identifying Hemoglobin variants in the screening for Hemoglobinopathies. It’s a cheaper alternative to the now preferred method of detection of Hb variants, i.e., HPLC. In this study, at a government hospital clinical biochemistry laboratory setup, we try to compare and emphasize the importance of this manual procedure. We have done this observation from the archives of lab and case sheet data for the past four and half years; that 1891 electrophoresis were done and 65% were normal, 20% shown Thalassemia trait, and 6% were Thalassemia major and 8% were shown sickle Hb pattern. Majority correlated clinically with the electrophoresis report and in doubtful cases (1.32%), HPLC confirmed the electrophoresis report. Hence wherever feasible, we recommend this manual alkaline agar gel electrophoresis where cost is a limiting factor.
Authors and Affiliations
Babu Rao Rentapalli, Jayalakshmi Gade, Md Dawood Sulemani, Suresh Babu Ganji, Pavani Kiranmai Thandu, Suneetha Revupalli, Sudhakar Kavali, Anitha Ramtenki
Keywords
Related Articles
- EP ID EP595107
- DOI 10.9790/0853-1604083943.
- Views 77
- Downloads 0
Autoimmune Diseases in Moroccan Population, About 5270 Cases Followed Between 2000 and 2016.
Background: Autoimmune diseases are a real public health problem. Any prevention policy cannot bemplemented without epidemiological studies. The aim of the present study is to describe the most common autoimmune diseases...
Level of Serum Lactate Dehydrogenase Enzyme in Oral Lichen Planus-A Biochemical Study
Aims and Objectives: Evaluation of alteration in serum LDH in patients with erosive lichen planus and to compare it with that of controls. Materials and Methods: Blood samples were collected using standardized methods in...
Effectiveness of Mannheim Peritonitis Index Scoring System in Predicitng the Morbidity And Mortality in Peritonitis Due To Hollow Viscous Perforation
Peritonitis is an inflammatory response of peritoneum to different stimuli caused by bacteria. It can also be fungal or chemical. Secondary peritonitis is due to spillage of gastrointestinal or genitourinary organisms in...
Study of Microbial Contaminationin Dental Unit Water Line: A Clinical Implication
Effective infection control is very crucial. The objective of this research is to determine the level of bacterial countin water from dental chair unit output at the Dental Clinic Faculty of Dentistry, UniversitiSains Is...
Study of Sleep Pattern And its Effects on Quality of Life Among Undergraduate Students in A Medical College of West Bengal
Aim: To assess the sleep pattern and burden of sleep problems among 1st year MBBS students. Materials and Methods: This cross-sectional study was conducted at GIMSH, Durgapur, West Bengal, India from February to April 20...