Agar Gel Alkaline Electrophoresis As A Cost Effective Screening Modality For Abnormal Hemoglobins
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 4
Abstract
Alkaline agar gel electrophoresis is a basic manual laborious technique for identifying Hemoglobin variants in the screening for Hemoglobinopathies. It’s a cheaper alternative to the now preferred method of detection of Hb variants, i.e., HPLC. In this study, at a government hospital clinical biochemistry laboratory setup, we try to compare and emphasize the importance of this manual procedure. We have done this observation from the archives of lab and case sheet data for the past four and half years; that 1891 electrophoresis were done and 65% were normal, 20% shown Thalassemia trait, and 6% were Thalassemia major and 8% were shown sickle Hb pattern. Majority correlated clinically with the electrophoresis report and in doubtful cases (1.32%), HPLC confirmed the electrophoresis report. Hence wherever feasible, we recommend this manual alkaline agar gel electrophoresis where cost is a limiting factor.
Authors and Affiliations
Babu Rao Rentapalli, Jayalakshmi Gade, Md Dawood Sulemani, Suresh Babu Ganji, Pavani Kiranmai Thandu, Suneetha Revupalli, Sudhakar Kavali, Anitha Ramtenki
Keywords
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- EP ID EP595107
- DOI 10.9790/0853-1604083943.
- Views 111
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