Albumin-like reactivity of amphoteric and cationic surfactants toward BCG color reagents
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 3
Abstract
Bromocresol Green (BCG) dyes exist as an equal mixture of undissociated (λmax 450 nm) and dissociated (λmax 620 nm) dye forms in acid solutions. In BCG color reagents for measuring serum albumin, color development of the dissociated dye is suppressed by added nonionic surfactants. Therefore, the BCG color reagents exhibit low absorbance levels of around 620 nm. The effects of different types of surfactants (SDS: anionic, Amphitol 24B: amphoteric, and Quartamin 24P: cationic) on the color development of BCG were investigated in this study. Our results revealed that absorbance levels of around 620 nm of the BCG color reagents were elevated by the addition of surfactants, especially those in amphoteric and cationic surfactants. These results indicated that amphoteric and cationic surfactants show albumin-like reactivity toward BCG color reagents.
Authors and Affiliations
Tomoko Arai, Toshihiko Tsukada
Keywords
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