Clinical sample comparison of eight homogeneous assay kits for high density lipoprotein cholesterol and low density lipoprotein cholesterol
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 2
Abstract
Homogeneous assay to measure the cholesterol associated with high- density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) are widely used in clinical laboratories all over the world. However, few reports have been published regarding the commutability of these tests for HDL-C and LDL-C. The aim of this study was to clarify the commutability of 8 homogeneous reagents currently used to measure HDL-C and LDL-C. The maximum coefficient of variation (CV) of the 990 health checkup samples measured with the 8 kits was 14.5% for HDL-C, whereas it was 26.9% for LDL-C. Of the 224 patient samples, high serum bilirubin levels affected the HDL-C values measured by one of the kits. High serum bilirubin levels and the IDL ratio affected the LDL-C values measured by some kits. Successive measurement revealed that the differences in the HDL-C and LDL-C values between laboratories were dependent on the difference in reactivity to lipoproteins. The homogeneous LDL-C measurements were significantly correlated with the Friedewald concentrations.
Authors and Affiliations
Yoshiaki Iizuka
Keywords
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